瓜蒌大黄汤对重症肺炎大鼠模型肺肠组织损伤的作用  

Effect of Gualou Dahuang Decoction on Lung and Intestinal Tissue Injury in Severe Pneumonia Rat Model

作　　者：李冠胜 吴倩[1] 张瑾[1] 王田田 邓扬嘉[1] 孙小平[1] Li Guansheng;Wu Qian;Zhang Jin;Wang Tiantian;Deng Yangjia;Sun Xiaoping(Chongqing Traditional Chinese Medicine Hospital,Chongqing 400011,China)

机构地区：[1]重庆市中医院,重庆400011

出　　处：《中国中医急症》2024年第3期419-423,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：重庆市自然科学基金面上项目(cstc2020jcyj-msxmX1010);重庆市科卫联合中医药技术创新与应用发展项目(2020ZY023689)。

摘　　要：目的观察瓜蒌大黄汤对重症肺炎大鼠模型肺肠组织损伤的作用。方法制备重症肺炎模型SD大鼠60只,随机分为盐水灌肠组、中药灌肠组,观察两组大鼠24、48、72 h 3个时间点的情况。再取10只健康SD大鼠作为空白组和10只重症肺炎模型大鼠作为模型组,造模成功后立即处死大鼠,采血并取肺、结肠组织。盐水灌肠组及中药灌肠组分别于造模成功后6、18、30、42、54、66 h进行盐水或中药灌肠,分别在24、48、72 h时处死大鼠,采血并取肺、结肠组织。HE染色观察肺肠组织病理损伤情况并评分;ELISA检测血清表面活性蛋白-A(SP-A)、降钙素原(PCT)、C反应蛋白(CRP);Western blotting、免疫荧光方法检测肺、肠组织中SP-A、肿瘤坏死因子-α(TNF-α)的表达。结果病理评分示:盐水灌肠组的肠组织病理评分高于各时间段中药灌肠组(P<0.05);盐水灌肠组的肺组织病理评分与中药灌肠组比较无统计学差异(P>0.05)。ELISA结果示:与空白组比较,模型组CRP、PCT、SP-A均升高(P<0.05);与模型组比较,盐水灌肠组血清CRP、PCT、SP-A各时间段呈升高趋势(P<0.05),中药灌肠组血清CRP、PCT变化不明显(P>0.05),SP-A在48 h至72 h升高(P<0.05);West⁃ern blotting和免疫荧光示:中药灌肠组肺、肠组织SP-A在各时间段呈升高趋势;盐水灌肠组肺、肠组织SP-A变化不明显。Western blotting结果示:与空白组比较,模型组肺、肠组织中TNF-α表达升高(P<0.05);与模型组比较,中药灌肠组肺组织TNF-α在72 h下降,而肠组织TNF-α在48、72 h表达下降(P<0.05);盐水灌肠组肺、肠组织TNF-α在48 h和72 h表达升高(P<0.05)。结论瓜蒌大黄汤灌肠可通过上调重症肺炎大鼠肺、肠组织SP-A的表达,下调TNF-α的表达,减轻炎性因子对肺、肠组织的损伤。Objective:To explore the effect of Gualou Dahuang Decoction on lung and intestinal tissue injury in severe pneumonia rat model.Methods:A total of 60 SD rats with severe pneumonia model were randomly divided into the saline enema group and TCM enema group with 30 rats in each group,and the two groups were further di⁃vided into three groups respectively:the 24 h group,48 h group and 72 h group.Then 10 healthy SD rats were se⁃lected as the control group,and 10 severe pneumonia model rats as the model group,which were immediately exe⁃cuted after successful modeling,and blood,lung and colon tissues were collected.The saline enema group and the TCM enema group were given saline or TCM enema at 6 h,18 h,30 h,42 h,54 h and 66 h after successful model⁃ing respectively.At last,the rats were executed at 24 h,48 h and 72 h,and blood and lung and colon tissues were collected.The injury of lung and intestine was observed and scored by HE staining of pathological sections;serum surfactant protein-A(SP-A),procalcitonin and C-reactive protein were detected by ELISA;expression of SP-A and TNF-αin lung and intestine tissues were detected by Western blotting and immunofluorescence.Results:Histopathological score:The intestinal histopathological score in the saline enema group was higher than that in the TCM enema group(P<0.05);the lung histopathological score in the saline enema group had no statistical dif⁃ference compared with the TCM enema group(P>0.05).ELISA results showed that compared with the control group,the levels of CRP,PCT and SP-A in the model group increased(P<0.05);compared with the model group,

关 键 词：重症肺炎 表面活性蛋白-A 瓜蒌大黄汤 灌肠 大鼠 

分 类 号：R285.5[医药卫生—中药学]