环洞庭湖土壤抗生素抗性基因分布和潜在风险  

Distributions and potential risks of antibiotic resistance genes in soils around Dongting Lake Basin,China

作　　者：武晨 黄凤莲 刘新刚 范青青 陆海楠 李启武 郭倩 李莹 彭勇春 李峰 WU Chen;HUANG Feng-lian;LIU Xin-gang;FAN Qing-qing;LU Hai-nan;LI Qi-wu;GUO Qian;LI Ying;PENG Yong-chun;LI Feng(College of Environment and Resources,Xiangtan University,Xiangtan 411105,China;Changsha Environmental Protection College,Changsha 410004,China;State Environmental Protection Engineering Center for Urban Soil Contamination Control and Remediation,Shanghai Academy of Environment Sciences,Shanghai 200233,China;The Key Laboratory for Environmental and Ecological Health in Hunan Province Ordinary Institutions of Higher Education,Xiangtan 411105,China;Changsha Ecological Environmental Monitoring Centre of Hunan Province,Changsha 410001,China;Hunan Ecological Environmental Monitoring Center,Changsha 410027,China;Xiangxi Ecological Environment Monitoring Center of Hunan Province,Jishou 416000,China;State Environmental Protection Key Laboratory of Monitoring for Heavy Metal Pollutants,Changsha 410014,China)

机构地区：[1]湘潭大学环境与资源学院,湖南湘潭411105 [2]长沙环境保护职业技术学院,湖南长沙410004 [3]国家环境保护城市土壤污染控制与修复工程技术中心,上海市环境科学研究院,上海200233 [4]环境生态健康湖南省普通高校重点实验室,湖南湘潭411105 [5]湖南省长沙生态环境监测中心,湖南长沙410001 [6]湖南省生态环境监测中心,湖南长沙410027 [7]湖南省湘西生态环境监测中心,湖南吉首416000 [8]国家环境保护重金属污染监测重点实验室,湖南长沙410014

出　　处：《中国环境科学》2024年第3期1575-1583,共9页China Environmental Science

基　　金：国家环境保护城市土壤污染控制与修复工程技术中心开放基金资助项目(USCR-202208);国家环境保护重金属污染监测重点实验室开放基金资助项目(SKLMHM202303);“新污染物的环境健康效应与协同治理技术”湖南省普通高等学校科技创新团队支持项目。

摘　　要：本研究通过实时荧光定量PCR和基于Monte Carlo模拟的概率健康风险评估模型,分析了环洞庭湖流域52个表层土壤点位中8类典型抗生素抗性基因(ARGs,16种ARGs亚型)的分布特征和潜在健康风险.结果表明,环洞庭湖流域周边表层土壤中ARGs总丰度范围为3.29×10^(7)~3.73×109copies/g,远高于土壤ARGs背景值(1.80×10^(5)~1.35×10^(7)copies/g,平均值为3.03×106copies/g).南洞庭湖流域表层土壤中ARGs丰度高于东、西洞庭湖流域.磺胺类、四环素类和氨基糖苷类ARGs是高丰度的ARGs类型,平均丰度分别为1.51×10^(8)、1.50×10^(7)和2.64×10^(7)copies/g,主要ARGs亚型是sul1和dfrA14,均属于磺胺类ARGs.I类整合子基因(intl1)的平均丰度为6.94×10^(8)copies/g,高于总ARGs平均丰度.相关性分析表明intl1丰度与dfrA14、tetR和kan基因丰度呈显著正相关(P<0.05),表明intl1通过水平基因转移促进了研究区域表层土壤中ARGs的传播.Monte Carlo模拟表明,研究区域存在64.92%~98.68%概率的ARGs人体暴露剂量高于风险背景值(成人12.56copies/(g·d),儿童38.11copies/(g·d)),表明研究区域土壤ARGs造成的潜在健康风险不可忽视,皮肤接触是ARGs主要暴露途径,磺胺类ARGs是潜在健康风险的主要来源.52 surface soil samples were collected around Dongting Lake basin.The distribution characteristics and potential health risks of eight typical antibiotic resistance genes(ARGs,comprising 16subtypes of ARGs)in surface soil were analyzed by real-time fluorescence quantitative PCR(qPCR)and a probability health risk assessment model based on Monte Carlo simulation.The total abundance of ARGs in surface soil of the Dongting Lake Basin ranged from 3.29×10^(7)to 3.73×109 copies/g,which was significantly higher than the soil background value of ARGs(1.80×10^(5)to 1.35×10^(7)copies/g,with an average of 3.03×106 copies/g).The southern Dongting Lake Basin exhibited higher ARGs abundance in surface soil compared to the eastern and western Dongting Lake Basin.Sulfonamide,tetracycline,and aminoglycoside resistance genes were the predominant ARGs,with average abundances of 1.51×10^(8),1.50×10^(7),and 2.64×10^(7)copies/g,respectively.The main subtypes of ARGs were sul1and dfrA14,both belonging to sulfonamide resistance genes.The average abundance of class I integron gene(intl1)was 6.94×10^(8)copies/g,higher than the average abundance of total ARGs in the study area.Correlation analysis showed a significant positive correlation(P<0.05)between intl1 abundance and the abundances of dfrA14,tetR,and kan genes,indicating that intl1promotes the spread of ARGs in surface soil via horizontal gene transfer.Monte Carlo simulation revealed that there was a probability of 64.92%to 98.68%for human exposure to ARGs exceeding risk background values(adults and children were 12.56 and 38.11copies/(g·d),respectively),indicating that the potential health risks associated with soil-borne ARGs in the study area.Skin contact was identified as the main exposure pathway for ARGs,and sulfonamide resistance genes were identified as the primary contributors to potential health risks of ARGs.

关 键 词：环洞庭湖 土壤 抗生素抗性基因 分布特征 潜在风险 

分 类 号：X53[环境科学与工程—环境工程]