蒙医温针调节神经递质及皮质醇含量治疗抑郁大鼠机制研究  

Study on the mechanisms of treating depression rats by regulating the neurotransmitter and cortisol content with Mongolian medicine warming acupuncture

作　　者：阿茹娜 包青林 赛音朝克图 阿古拉 斯楞格 ARUNA;BAO Qinglin;SAIYINCHAOKETU;AGULA;SILENGGE(Mongolian Medicine College,Inner Mongolia Medical University,Hohhot 010110,China;International Mongolian Hospital of Inner Mongolia,Hohhot 010013,China;Baotou Medical College,Inner Mongolia University of Science&Technology,Baotou 014040,China)

机构地区：[1]内蒙古医科大学蒙医药学院,呼和浩特010110 [2]内蒙古自治区国际蒙医医院,呼和浩特010013 [3]内蒙古科技大学包头医学院,内蒙古包头014040

出　　处：《吉林中医药》2024年第3期334-338,共5页Jilin Journal of Chinese Medicine

基　　金：内蒙古医科大学面上项目(YKD2021MS049);内蒙古自治区卫健委医疗卫生科技计划项目(202201212)。

摘　　要：目的 观察蒙医温针对抑郁大鼠中枢氨基酸类神经递质含量的影响,观察蒙医温针对应激条件下大鼠的焦虑症状的调节作用,探讨其治疗抑郁症的机理。方法 45只清洁级SD大鼠随机分为蒙医温针组、西药治疗组和生理盐水组,采用“慢性不可预知应激(CUMS)”制作大鼠抑郁模型,分别在0 h,24 h,48 h和72 h时,记录每个时段大鼠体质量,之后采用“高效液相色谱荧光检测法”检测中枢氨基酸类神经递质;并采血检测血清皮质醇变化。结果 抑郁模型大鼠中海马及下丘脑的γ-氨基丁酸(GABA)在蒙医温针组要低于生理盐水组(P <0.01,P <0.05);海马及下丘脑谷氨酸含量在蒙医温针组要高于生理盐水组(P <0.01,P <0.05)。蒙医温针组治疗后皮质醇含量高于西药治疗组(P <0.01)。结论 蒙医温针通过调节中枢氨基酸类神经递质水平改善抑郁症,蒙医温针治疗可以减轻抑郁大鼠的焦虑症状。Objective To observe the effects of Mongolian medicine warming acupuncture on the content of central amino acid neurotransmitters in rats with a depressive disorder,explore its mechanisms on treating the disease,and observe its effect in the regulation of anxiety symptoms in rats under stress conditions.Methods A total of 45 clean SD rats were randomly divided into an acupuncture group,a western medicine group and a normal saline group.Chronic unpredictable mild stress(CUMS)was used to establish a rat depression model.Their body weights were recorded at Oh,24h,48h and 72h,respectively,and then the central amino acid neurotransmitters were detected by the high performance liquid chromatography fluorescence detection method,and their blood samples were collected to detect the changes in serum cortisol.Results After treatment,the levels of-aminobutyric acid(GABA)in hippocampus and hypothalamus of depression model rats were significantly lower in the warming acupuncture group than those in the normal saline group(P<0.01,P<0.05).The contents of glutamate in hippocampus and hypothalamus in the warming acupuncture group were significantly higher than those in the normal saline group(P<0.01,P<0.05).The content of cortisol in the acupuncture group was significantly higher than that in the western medicine group(P<0.01).Conclusion Mongolian medicine warming acupuncture can improve a depressive disorder by regulating the level of central amino acid neurotransmitters,and alleviate anxiety symptoms in rats with the disease.

关 键 词：抑郁症 蒙医温针 中枢氨基酸类神经递质 皮质醇 

分 类 号：R29[医药卫生—民族医学]