基于SNP标记的小麦品种遗传相似度及其检测准确度分析  

Genetic similarity and its detection accuracy analysis of wheat varieties based on SNP markers

作　　者：许乃银[1] 金石桥[2] 晋芳[2] 刘丽华[3] 徐剑文[1] 刘丰泽 任雪贞 孙全 许栩 庞斌双[3] XU Nai-Yin;JIN Shi-Qiao;JIN Fang;LIU Li-Hua;XU Jian-Wen;LIU Feng-Ze;REN Xue-Zhen;SUN Quan;XU Xu;PANG Bin-Shuang(Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Jiangsu,China;National Agricultural Technical Extension and Service Center,Beijing 100125,China;Institute of Hybrid Wheat,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)

机构地区：[1]江苏省农业科学院经济作物研究所,江苏南京210014 [2]全国农业技术推广服务中心,北京100125 [3]北京市农林科学院杂交小麦研究所,北京100097

出　　处：《作物学报》2024年第4期887-896,共10页Acta Agronomica Sinica

基　　金：国家科技创新重大项目(2022ZD04019)资助。

摘　　要：遗传相似度检测的准确度估计是对SNP标记法在农作物品种检测体系中应用的必要补充和完善。本研究基于2021年小麦品种SNP标记法跨实验室协同验证实验数据,分析了该方法的检测准确度及在品种间的遗传相似度。分析结果表明:(1)10个实验室对55组小麦品种组合的标记位点相似度检测的总体准确度约为98%。(2)GGE双标图的品种遗传关系功能图显示,7组小麦品种的组内遗传相似度在95%以上,其余组合的遗传相似度较低。(3)依据GGE双标图的“正确度-精确度”功能图和“准确度排序”功能图,发现洛旱7号/洛旱11等品种组合的相似度检测准确度较高,晋麦47/临抗11的检测准确度一般,而济麦22/婴泊700的检测准确度较差。(4)10个实验室的检测准确度存在显著差异,其中2个实验室检测的正确度、精确度和准确度表现显著差于其余实验室。(5)各实验室检测正确度的容许误差分布于1.3%~1.9%之间,平均为1.5%;准确度的容许误差分布于1.5%~2.0%之间,平均为1.7%。其中,Lab2和Lab3的检测正确度和准确度的容许误差显著差于其余实验室。本研究构建了SNP标记法对品种相似性检测的准确度统计模型,分析了品种组合和实验室的检测准确度及其容许误差,采用GGE双标图方法对检测正确度、精确度和准确度进行可视化分析,验证了各实验室对品种位点相似性检测的准确度和可靠性,为SNP标记法在农作物品种遗传相似性检测中的准确度评价提供了理论支持和应用范例。The accuracy estimation of genetic similarity detection of crop varieties is an indispensable supplement and improvement to the application of SNP marker method in crop variety detection technology system.In this study,based on the cross-laboratory collaborative validation test data using SNP molecular marker method in 2021,the genetic similarity among wheat varieties and the accuracy of SNP molecular marker method in variety similarity detection were analyzed.The results showed as follows:(1)The overall accuracy of marker locus similarity detection among 55 wheat variety combinations by 10 laboratories was approximately 98%.(2)The genetic relationship between varieties view of GGE biplot delineated the genetic relationship between varieties.The genetic similarity between seven combinations of wheat varieties was over 95%,and the genetic similarity of other combinations was relatively lower.(3)The“trueness-precision”view and“accuracy ranking”view of GGE biplot identified that the similarity detection accuracy of the variety combination Jinmai 47/Linkang 11 was on average,Jimai 22/Yingbo 700 was relatively lower,while Luohan 7/Luohan 11 and other variety combinations were relatively high.(4)Significant differences were existed in detection accuracy among the 10 laboratories,and the performances in detection trueness,precision and accuracy of two laboratories were significantly worse than those of other laboratories.(5)The tolerance error of the trueness of each laboratory ranged from 1.3%to 1.9%,with an average of 1.5%.The tolerance error of accuracy was distributed between 1.5%and 2.0%,with an average of 1.7%.Among them,the tolerance errors of the detection trueness and accuracy of Lab2 and Lab3 were significantly worse than those of the other laboratories.In this study,the detection accuracy statistical model of SNP marker method in detecting crop variety similarity was constructed to analyze the detection accuracy and the corresponding tolerance error of variety combination in different laboratories,and

关 键 词：小麦(Triticum aestivum L.) GGE双标图 SNP标记 遗传相似度 位点相似度 准确度 

分 类 号：S512.1[农业科学—作物学]