基于转录组探究外源水杨酸对条锈菌侵染小麦幼苗的缓解效应及差异表达基因分析  

Alleviative effect of salicylic acid on wheat seedlings with stripe rust based on transcriptome and differentially expressed genes

作　　者：齐学礼 李莹 李春盈 韩留鹏 赵明忠 张建周[3] QI Xue-Li;LI Ying;LI Chun-Ying;HAN Liu-Peng;ZHAO Ming-Zhong;ZHANG Jian-Zhou(Crops Molecular Breeding Academy of Henan,Zhengzhou 450002,Henan,China;Editorial Department of Journal of Henan Agricultural University,Zhengzhou 450002,Henan,China;Wheat Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China)

机构地区：[1]河南省作物分子育种研究院,河南郑州450002 [2]河南农业大学学报编辑部,河南郑州450002 [3]河南省农业科学院小麦研究所,河南郑州450002

出　　处：《作物学报》2024年第4期1080-1090,共11页Acta Agronomica Sinica

基　　金：财政部和农业农村部国家现代农业产业技术体系建设专项(小麦,CARS-3-7);河南省农业科学院自主创新项目(2022ZC03)资助。

摘　　要：为探究外源水杨酸提高小麦条锈病抗性的作用机制,本文将周麦18分为未处理的小麦幼苗(对照)、条锈菌侵染的小麦幼苗(SR)和水杨酸处理条锈菌侵染的小麦幼苗(SA-SR),对处理15 d后的小麦幼苗进行抗病性鉴定、氨基酸含量检测、转录组测序及荧光定量分析鉴定。结果表明:外源水杨酸能够显著降低条锈菌对小麦幼苗的致病力;SR组叶片中氨基酸含量普遍降低,而经过水杨酸预先处理的SA-SR组与Control无显著差异,说明小麦条锈病造成叶片中氨基酸的含量显著降低;通过GO富集分析发现,SR和SA-SR组的差异基因在与光合作用相关的生物过程中富集,涉及的细胞组分包括叶绿体和类囊体等,KEGG通路富集分析显示,SR和SA-SR两个分组均在MAPK信号通路和次生代谢物的生物合成中显著富集,在植物的抗病或抗逆中发挥重要作用。In order to explore the mechanism of exogenous salicylic acid in improving the resistance of wheat stripe rust,using,the untreated wheat seedlings(control),stripe rust wheat seedlings(SR),and salicylic acid treated stripe rust wheat seedlings(SA-SR)as the experimental materials were investigated using Zhoumai 18.After 15 days of treatment,the wheat seedlings were identified for disease resistance,amino acid detection,transcriptome sequencing,and fluorescence quantification.The results indicated that the exogenous salicylic acid could significantly reduce the pathogenicity of stripe rust in wheat seedlings.The content of amino acids in leaves in the SR group was generally reduced,while there was no significant difference between the SA-SR group and the control group pretreated with salicylic acid,indicating that the content of amino acids in leaves was significantly reduced due to wheat stripe rust.GO enrichment indicated that the differentially expressed genes of SR and SA-SR groups were enriched in biological processes related to photosynthesis involving cell components such as chloroplasts and thylakoids.KEGG pathway enrichment showed that both SR and SA-SR subgroups were significantly enriched in the MAPK signaling pathway and the biosynthesis of secondary metabolites played an important role in plant disease resistance or stress resistance.

关 键 词：小麦条锈病 水杨酸 氨基酸 转录组 

分 类 号：S435.121.42[农业科学—农业昆虫与害虫防治]