重症毛细支气管炎患儿血清Presepsin、TSP-1表达及其临床意义  

A Study of the Expression and Clinical Significance of Serum Presepsinand TSP-1 in Children with Severe Bronchiolitis

作　　者：刘严 李强 张怀坤 LIU Yan;LI Qiang;ZHANG Huaikun(Department of Pediatrics,Huaibei Maternal and Child Health Hospital,Huaibei 235000,China)

机构地区：[1]淮北市妇幼保健院儿内科,安徽淮北235000

出　　处：《标记免疫分析与临床》2023年第12期2112-2116,共5页Labeled Immunoassays and Clinical Medicine

基　　金：2017年度蚌埠医学院自然科学基金重点项目(编号:BYKY1748ZD)。

摘　　要：目的探讨重症毛细支气管炎患儿血清可溶性白细胞分化抗原14亚型(Presepsin)、凝血酶敏感蛋白1(TSP1)表达水平及临床意义。方法选择2021年11月至2023年1月我院收治的102例毛细支气管炎患儿为研究对象(研究组),根据病情严重程度将患儿分为重症组(30例)和非重症组(72例),另选取同期在我院进行健康体检的50例婴幼儿为对照组。采用酶联免疫吸附法(ELISA)对各组婴幼儿血清Presepsin、TSP1水平进行测定;Spearman相关性分析患儿血清Presepsin、TSP1水平与病情之间的关系;Logistic回归分析影响重症毛细支气管炎发生的影响因素;受试者工作特征曲线(ROC)评估Presepsin、TSP1对重症毛细支气管炎的诊断价值。结果与对照组相比,研究组患儿血清Presepsin、TSP1均显著升高(P<0.05),且重症组患儿血清Presepsin、TSP1水平均显著高于非重症组(P<0.05);Spearman相关性分析显示,血清Presepsin、TSP1水平与病情程度均呈显著正相关(r 1=0.593,r 2=0.425,P<0.05);Logistic回归分析结果显示,Presepsin、TSP1是影响重症毛细支气管炎发生的危险因素(P<0.05);ROC曲线结果显示,Presepsin、TSP1单独诊断重症毛细支气管炎的AUC分别为0.861、0.771,灵敏度分别为62.5%、65.6%,特异性分别为61.1%、40.6%,二者联合诊断重症毛细支气管炎的AUC为0.951,灵敏度为90.6%,特异性为80.9%。结论重症毛细支气管炎患儿血清Presepsin、TSP1表达水平显著升高,且血清Presepsin、TSP1水平与病情程度呈正相关,两者可为早期诊断病情和临床治疗提供参考。Objective To investigate the expression levels and clinical significance of serum soluble leukocyte differentiation antigen subtype 14(Presepsin)and thrombin sensitive protein 1(TSP1)in children with severe bronchiolitis.Methods A total of 102 children with bronchiolitis admitted to our hospital from November,2021 to January,2023 were collected as the research subjects(study group).According to the severity of the condition,these children were grouped into a severe group(30 cases)and a non-severe group(72 cases),while another 50 infants and young children who underwent health examinations in our hospital were collected as the control group.Enzyme-linked immunosorbent assay(ELISA)was applied to determine serum levels of Presepsin and TSP1 in infants and young children in each group;Spearman correlation was applied to analyze the relationship between serum levels of Presepsin and TSP1 in pediatric patients and their condition;Logistic regression was applied to analyze the influencing factors of severe bronchiolitis;Receiver operating characteristic curve(ROC)was drawn to evaluate the diagnostic value of Presepsin and TSP1 in severe bronchiolitis.Results Compared with the control group,serum levels of Presepsin and TSP1 in the study group were significantly increased(P<0.05).Furthermore,serum levels of Presepsin and TSP1 in the critically ill group were significantly higher than those in the non-critically ill group(P<0.05);Spearman correlation analysis showed that serum levels of Presepsin and TSP1 were significantly positively correlated with the severity of the disease(r 1=0.593,r 2=0.425,P<0.05);Logistic regression analysis showed that Presepsin and TSP1 were risk factors affecting the occurrence of severe bronchiolitis(P<0.05);ROC curve results showed that AUC of Presepsin and TSP1 in diagnosing severe bronchiolitis were 0.861 and 0.771,respectively,with sensitivity of 62.5%and 65.6%,specificity of 61.1%and 40.6%,respectively,while AUC of the combined diagnosis of severe bronchiolitis was 0.951,with a sensitivi

关 键 词：重症毛细支气管炎 可溶性白细胞分化抗原14亚型 凝血酶敏感蛋白1 病情 

分 类 号：R725.6[医药卫生—儿科]