益脾养肝方对肝癌前病变大鼠肝星状细胞活化的影响  

Effect of Yipi Yanggan Formula on the Activation of Hepatic Stellate Cells in Rats with Pre Liver Cancer Lesions

作　　者：鞠迪[1] 李汨 韩曼[1] 房冰莹 闫曙光[1] 安荣[1] 李京涛 JU Di;LI Mi;HAN Man;FANG Bingying;YAN Shuguang;AN Rong;LI Jingtao(Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712046,China;Affiliated Hospital of Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712000,China)

机构地区：[1]陕西中医药大学,陕西咸阳712046 [2]陕西中医药大学附属医院,陕西咸阳712000

出　　处：《陕西中医药大学学报》2024年第2期83-88,共6页Journal of Shaanxi University of Chinese Medicine

基　　金：国家自然科学基金项目(81904192);陕西省教育厅项目(19JK0244);陕西中医药大学科学研究计划项目(2017QN15);陕西中医药大学学科创新团队项目(2019-YS06);陕西省自然科学基础研究计划青年项目(2022JQ-793,2022JQ-870);陕西省中医药管理局项目(15-JC004)。

摘　　要：目的探讨益脾养肝方对二乙基亚硝胺(Diethylnitrosamine,DEN)诱导的肝癌前病变中肝星状细胞活化及胶原沉积的影响。方法将26只雄性Sprague-Dawley大鼠随机分为空白组、模型组、益脾养肝方组和护肝片组,空白组5只,其余3组各7只。腹腔注射DEN诱导肝癌前病变模型,给药14 w后处死。取肝组织观察并记录其大小、外观等变化,计算肝重比(肝脏指数),通过HE染色观察大鼠肝组织病理形态学改变,天狼星红染色观察各组胶原沉积表达,免疫组化检测α-平滑肌动蛋白(alpha smooth muscle actin,α-SMA)的表达,实时定量PCR(Real Time PCR,RT-PCR)检测Ⅰ型胶原蛋白(typeⅠcollagen,CollagenⅠ)的表达,以研究益脾养肝方对大鼠肝癌前病变模型的影响。计量资料多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验。结果与模型组相比,益脾养肝方组和护肝片组肝脏病理形态显著改善,肝脏指数、CollagenⅠ和α-SMA表达均降低(P值均<0.05);与护肝片组相比,益脾养肝方组对肝脏的保护作用更显著,其肝脏指数、CollagenⅠ和α-SMA表达均显著降低(P值均<0.05)。结论益脾养肝方可有效改善DEN诱导的大鼠肝癌前病变,其机制可能与抑制肝星状细胞活化,减少胶原沉积有关。Objective To explore the effects of Yipi Yanggan formula on the activation of hepatic stellate cells and collagen deposition in diethylnitrosamine(DEN)induced precancerous lesions of liver cancer.Methods 26 male Sprague Dawley rats were randomly divided into a blank group,a model group,a Yipi Yanggan formula group,and a Hugan tablet group.There were 5 rats in the blank group and 7 rats in each of the other 3 groups.Den was injected intraperitoneally to induce a model of liver cancer precancerous lesions,and was euthanized after 14 weeks of administration.Take liver tissue for observation and record changes in size,appearance,etc.,calculate liver weight ratio(liver index),observe pathological and morphological changes in rat liver tissue through HE staining,observe collagen deposition and expression in each group through Sirius red staining,detect the expression of alpha smooth muscle actin(α-SMA)through immunohistochemistry,and detect typeⅠcollagen(CollagenⅠ)through real-time quantitative PCR(RT-PCR)To investigate the effect of Yipi Yanggan formula on the pre liver cancer lesion model in rats.Multiple inter group comparisons of quantitative data were conducted using one-way analysis of variance,and further pairwise comparisons were conducted using LSD-t test.Results Compared with the model group,the liver pathological morphology of the Yipi Yanggan formula group and the Hugan tablet group was significantly improved,and the liver index,Collagen I,andα-SMA expression were all reduced(P<0.05);Compared with the liver protection tablet group,the Yipi Yanggan formula group has a more significant protective effect on the liver,with significantly reduced liver index,Collagen I,andα-SMA expression(P<0.05).Conclusion Yipi Yanggan formula can effectively improve DEN induced precancerous lesions in rats,and its mechanism may be related to inhibiting hepatic stellate cell activation and reducing collagen deposition.

关 键 词：肝癌前病变 益脾养肝方 肝星状细胞 Α-平滑肌动蛋白 胶原沉积 

分 类 号：R256.4[医药卫生—中医内科学]