Rapid accuracy determining DNA purity and concentration in heavy oils by spectrophotometry methods  

作　　者：YunYang Wan HongMei Mu Na Luo JianPing Yang Yan Tian Ning Hong HaiLiang Dong 

机构地区：[1]National Key Laboratory of Petroleum Resources and Engineering,Research Centre for Geomicrobial Resources and Application,Beijing Key Laboratory of Petroleum Pollution and Control,Unconventional Petroleum Research Institute,China University of Petroleum,Beijing,102249,China [2]Drilling and Production Engineering Technology Department of PetroChina Liaohe Oilfield Company,Panjin,124010,Liaoning,China [3]Center for Geomicrobiology and Biogeochemistry Research,State Key Laboratory of Biology and Environmental Geology,China University of Geosciences,Beijing,100083,China

出　　处：《Petroleum Science》2023年第6期3394-3399,共6页石油科学（英文版）

基　　金：supported by grants from the PetroChina-CUP Major Strategic Cooperation Projects(ZLZX2020010805,ZLZX2020020405);National Natural Science Foundation of China(41373086);National Science and Technology Major Project(No.2016ZX05050011,2016ZX05040002);Beijing Nova Program and Leading Talent Culturing Cooperative Projects(No.Z161100004916033);Beijing Higher Education Young Elite Teacher Project(No.YETP0670);Outstanding Young Excellent Teachers Foundation of China University of Petroleum(Beijing)(KYJJ2012-01-10).

摘　　要：DNA analysis is the core of biotechnology applied in petroleum resources and engineering. Traditionally accurate determination of DNA purity and concentration by spectrometer is the first and critical step for downstream molecular biology research. In this study, three different spectrophotometry methods, BPM, NDTT and NPMTTZ were compared for their performance in determining DNA concentration and purity in 32 oil samples, and molecule methods like quantitative real-time PCR (qPCR) and high-throughput sequence were also performed to help assess the accuracy of the three methods in determining DNA concentration and purity. For ordinary heavy oil (OHO), extra heavy oil (EHO) and super heavy oil (SHO), the characteristics of high viscosity (η), density (ρ) and resin plus asphaltene content will affect the DNA extraction and UV determination. The DNA concentration was decreased as density increased: OHO (11.46 ± 18.34 ng/μL), EHO (6.68 ± 9.67 ng/μL) and SHO (6.20 ± 7.83 ng/μL), and the DNA purity was on the reverse: OHO (1.31 ± 0.27), EHO (1.54 ± 0.20), and SHO (1.83 ± 0.32). The results suggest that spectrophotometry such as BPM and NPMTTZ are qualitatively favorite methods as the quick non-consumable methods in determining DNA concentration and purity of medium oil and heavy oil.

关 键 词：Heavy oil DNA concentration DNA purity SPECTROPHOTOMETRY qPCR 

分 类 号：TE621[石油与天然气工程—油气加工工程]