免疫性血小板减少症儿童外周血miRNA-150及血小板膜糖蛋白特异性抗体的表达及临床意义  

Expression and clinical value of miRNA-150 and platelet membrane glycoprotein specific antibodies in peripheral blood of children with immune thrombocytopenia

作　　者：张学会 韩晶晶 郭燕 刘瑞寒 Zhang Xuehui;Han Jingjing;Guo Yan;Liu Ruihan(Department of Pediatrics,the Affiliated Hospital of Jining Medical College,Jining 272000,China)

机构地区：[1]济宁医学院附属医院儿科,山东济宁272000

出　　处：《国际免疫学杂志》2023年第6期630-635,共6页International Journal of Immunology

摘　　要：目的分析免疫性血小板减少症(imumune thrombocytopenia,ITP)儿童外周血中miRNA-150以及血小板膜糖蛋白(glycoprotein,Gp)特异性抗体表达情况和临床意义。方法选择2020年1月至2022年12月济宁医学院附属医院儿科收治的ITP患儿总共98例设为观察组,另选择同时间济宁医学院附属医院儿科健康体检儿童100例设为对照组,采用实时荧光定量反应检测并比较两组研究对象外周血miRNA-150以及Gp特异性抗体水平,分析观察组不同疾病分期和不同疗效患儿外周血中miRNA-150水平及有关抗体表达情况。结果观察组患儿外周血miRNA-150水平高于对照组[(1.14±0.30)比(0.39±0.05),t=24.65,P<0.05]。观察组中新发ITP组的miRNA-150水平、抗GPIIb/Ⅲa抗体阳性率高于持续性ITP组以及慢性ITP组[(1.24±0.22)比(1.15±0.20)、(0.88±0.16),75.00%比54.05%、61.90%,F/χ^(2)值为14.35、11.38,P值均<0.05]。观察组中治疗后显效56例,有效21例,无效21例。显效组的miRNA-150水平、抗GPIIb/Ⅲa抗体阳性率均低于有效组以及无效组(0.66±0.14)比(0.98±0.23)、(1.20±0.35),12.50%比47.62%、80.95%,F/χ^(2)值为10.68、6.50,P值均<0.05),抗GPIIb/Ⅲa、抗GPIb/Ⅸ均为阴性的比例高于有效组以及无效组[83.93%比(38.10%、4.76%),P<0.05]。结论ITP儿童外周血中miRNA-150呈高表达状态,且新发患者miRNA-150、抗GPIIb/Ⅲa抗体阳性率更高,miRNA-150以及在Gp特异性抗体在评估ITP患儿疾病分期以及疗效中具有重要价值。Objective To analyze the expression and clinical value of miRNA-150 and platelet membrane glycoprotein(Gp)specific antibodies in peripheral blood of children with immune thrombocytopenia(ITP).Methods A total of 98 children with ITP admitted to the affiliated hospital of jining medical college from January 2020 to December 2022 were selected as the observation group,and a total of 100 healthy children undergoing physical examination in our hospital during the same period were selected as the control group.The levels of miRNA-150 and Gp specific antibodies in peripheral blood were detected,and the levels of miRNA-150 in peripheral blood of the two groups were compared.The levels of miRNA-150 and related antibodies in peripheral blood of children with different disease stages and different therapeutic effects in the observation group were compared.Results The level of miRNA-150 in the peripheral blood of the observation group was higher than that of the control group[(1.14±0.30)vs(0.39±0.05),t=24.65,P<0.05].In the observation group,the miRNA-150 level and the positive rate of anti GPIIb/Ⅲa antibody in the newly diagnosed ITP group were higher than those in the persistent ITP group and the chronic ITP group[(1.24±0.22)vs(1.15±0.20),(0.88±0.16),75.00%vs 54.05%,61.90%,F/χ^(2) values were 14.35 and 11.38,both P values<0.05].In the observation group,there were 56 cases of complete reactions after treatment,21 cases of effective reactions,and 21 cases of ineffective reactions.The miRNA-150 level and positive rate of anti GPIIb/Ⅲa antibodies in the complete response group were lower than those in the effective and ineffective groups(0.66±0.14)vs(0.98±0.23),(1.20±0.35),12.50%vs 47.62%,80.95%,F/χ^(2) values were 10.68 and 6.50,both P values<0.05),the positive rates of negative anti GPIIb/Ⅲa and anti GPIb/Ⅸwere higher than those of the effective and ineffective groups[83.93%vs(38.10%,4.76%),P<0.05].Conclusion The expression of miRNA-150 in the peripheral blood of children with ITP is high,and the positive ra

关 键 词：免疫性血小板减少症 miRNA-150 血小板膜糖蛋白 特异性抗体 疾病分期 

分 类 号：R725.5[医药卫生—儿科]