Single-cell RNA sequencing reveals a hierarchical transcriptional regulatory network of terpenoid biosynthesis in cotton secretory glandular cells  

作　　者：Jia-Ling Lin Longxian Chen Wen-Kai Wu Xiao-Xiang Guo Cheng-Hui Yu Min Xu Gui-Bin Nie Jun-ling Dun Yan Li Baofu Xu Ling-Jian Wang Xiao-Ya Chen Wei Gao Jin-Quan Huang 

机构地区：[1]State Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Shanghai Institute of Plant Physiology and Ecology,University of CAS,Chinese Academy of Sciences,Shanghai 200032,China [2]School of Life Science and Technology,ShanghaiTech University,Shanghai 200031,China [3]Chongqing Key Laboratory of Micro-Nano Systems and Intlligent Transduction,Collaborative Innovation,National Research Base of Inteligent Manufacturing Service,Chongqing Technology and Business University,Chongqing 400067,China [4]Analytical Applications Center,Shimadzu(China)Co.,Ltd.,Shanghai 200233,China [5]Shandong Laboratory of Yantai Drug Discovery,Bohai Rim Advanced Research Insttute for Drug Discovery,Yantai 264117,Shandong,China [6]State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China [7]Shanghai Key Laboratory of Plant Functional Genomics and Resources,Shanghai Chenshan Botanical Garden,Shanghai 201602,China [8]National Key Laboratory of Cotton Bio-breeding and Integrated Utilization(Henan University),Henan 475004,China

出　　处：《Molecular Plant》2023年第12期1990-2003,共14页分子植物（英文版）

基　　金：the National Key R&D Program of China(2022YFF1001400)to J.-Q.H.;the National Natural Science Foundation of China(32388201)to X.-Y.C.;the Chinese Academy of Sciences(XDB27020207)to X.-Y.C.;the Foundation of Youth Innovation Promotion Association of the Chinese Academy of Sciences to J.-Q.H.;the Young Elite Scientists Sponsorship Program by CAST(2019QNRC001)to J.-Q.H.;the Yunnan Revitalization Talent Support Program"Top Team"Project(202305AT350001)to X.-Y.C.;Winall Hi-tech Seed Co.,Ltd.(GMLM2023)to X.-Y.C.;the National Natural Science Foundation of China(32172041)to W.G.

摘　　要：Plants can synthesize a wide range of terpenoids in response to various environmental cues.However,the specific regulatory mechanisms governing terpenoid biosynthesis at the cellular level remain largely elusive.In this study,we employed single-cell RNA sequencing to comprehensively characterize the transcriptome profile of cotton leaves and established a hierarchical transcriptional network regulating cellspecific terpenoid production.We observed substantial expression levels of genes associated with the biosynthesis of both volatile terpenes(such asβ-caryophyllene andβ-myrcene)and non-volatile gossypol-type terpenoids in secretory glandular cells.Moreover,two novel transcription factors,namely GoHSFA4a and GoNAC42,are identified to function downstream of the Gossypium PIGMENT GLAND FORMATION genes.Both transcription factors could directly regulate the expression of terpenoid biosynthetic genes in secretory glandular cells in response to developmental and environmental stimuli.For convenient retrieval of the single-cell RNA sequencing data generated in this study,we developed a user-friendly web server.Our findings not only offer valuable insights into the precise regulation of terpenoid biosynthesis genes in cotton leaves but also provide potential targets for cotton breeding endeavors.

关 键 词：HIERARCHICAL network COTTON 

分 类 号：Q94[生物学—植物学]