广东省大豆农家种芽期耐盐性鉴定  

11Identification of salt tolerance of soybean landraces at germination stage in Guangdong

作　　者：刘双幸 赖剑云 李红梅 陈沛 吉浴芳 张文虎 马启彬[2] 贾俊婷 刘军 LIU Shuang-xing;LAI Jian-yun;LI Hong-mei;CHEN Pei;JI Yu-fang;ZHANG Wen-hu;MA Qi-bin;JIA Jun-ting;LIU Jun(Agro-biological Gene Research Center,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization,Guangzhou,Guangdong 510640,China;College of Agricul‐ture,South China Agricultural University,Guangzhou,Guangdong 510642,China;College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225,China)

机构地区：[1]广东省农业科学院农业生物基因研究中心/广东省农作物种质资源保存与利用重点实验室,广东广州510640 [2]华南农业大学农学院,广东广州510642 [3]仲恺农业工程学院农业与生物学院,广东广州510225

出　　处：《南方农业学报》2023年第11期3228-3238,共11页Journal of Southern Agriculture

基　　金：广东省重点领域研发计划项目(2020B020220008,2022B0202110003,2020B020220002)。

摘　　要：【目的】鉴定广东省大豆农家种芽期耐盐性,发掘广东省大豆农家种中潜在的优异性状,筛选出耐盐性较强的种质资源,为耐盐品种的改良和培育提供优质亲本材料。【方法】以102份广东农家大豆种质资源为试验材料,用150 mmol/L NaCl进行盐胁迫处理,以蒸馏水为对照,处理后测定发芽势、发芽率、盐害指数、胚轴长、胚根长、鲜重和干重,根据盐害指数进行耐盐性初步分级,计算其余指标相对值并进行相关分析,采用主成分分析法和隶属函数法计算综合评价值D,并进行耐盐性排序,使用聚类分析法筛选耐盐性较强的种质资源。【结果】耐盐性初步分级结果显示,102份种质资源中,高耐种质资源占比最多,较耐种质资源次之,中耐和较敏感种质资源占比相同,无敏感种质资源。与对照相比,盐胁迫下大豆芽期指标均降低,其受抑制程度排序为胚根长>胚轴长>鲜重>干重>发芽势>发芽率,芽期胚根长受抑制作用较大,发芽率受抑制作用较小;相关分析结果显示,多数指标间均存在一定程度的相关性;通过主成分分析得到2个主成分,累积贡献率达88.17%,其中第一主成分(PC1)中特征向量绝对值最大的指标为相对发芽势,第二主成分(PC2)中特征向量绝对值最大的指标为相对发芽率和盐害指数;采用获得的2个主成分作为综合指标,根据D值进行耐盐性排序,排名前5的种质资源编号分别为374、310、358、357和309;通过聚类分析筛选出耐盐性较强的种质资源,编号分别为310、358、374和331。【结论】筛选出4份耐盐性较强的农家大豆种质资源,可作为亲本材料用于耐盐种质创制,或者作为抗盐渍种质资源用于盐渍化土地的开发利用。相对发芽势和相对发芽率指标可用于大豆耐盐性评价与耐盐种质资源筛选。【Objective】The purpose of the study was to identify the salt tolerance of soybean landraces at germination stage in Guangdong,to discover the potential excellent traits in soybean landraces in Guangdong,to screen out germ‐plasms with strong salt tolerance,and to provide high-quality parent materials for the improvement and cultivation of salt tolerant varieties.【Method】A total of 102 Guangdong soybean landrace germplasm resources were selected as the experi‐mental materials,and the germination potential,germination rate,salt injury index,hypocotyl length,radicle length,fresh weight and dry weight were measured after treatment with 150 mmol/L NaCl for salt stress,and distilled water was used as the control.The preliminary classification of salt tolerance was carried out by the salt injury index.The relative values of the remaining indexes were calculated and correlation analysis was performed.The principal component analysis method and membership function method were used to calculate the comprehensive evaluation D value,and the salt tole-rance was ranked.Germplasms with strong salt tolerance were screened by cluster analysis method.【Result】According to the preliminary classification result of salt injury index,it was found that highly tolerant germplasm resources accounted for the largest proportion of the 102 germplasm resources,followed by tolerant germplasm resources,moderately tolerant and sensitive germplasm resources accounted for the same proportion,there was no sensitive germplasm resource.Com‐pared with the control,the indexes of soybean germination stage were all reduced under salt stress,and their degree of in‐hibition was in the order of radicle length>hypocotyl length>fresh weight>dry weight>germination potential>germination rate,and radicle length at germination stage was more inhibited,while germination rate was less inhibited.The results of correlation analysis showed that there was a certain degree of correlation between most of the indexes.Through principal component analysis

关 键 词：大豆 农家品种 种质资源 芽期 耐盐鉴定 

分 类 号：S565.1[农业科学—作物学]