Compound fufangteng mixture affects the expression of CD69 on CD11b^(+) monocytes in immunosuppressed mice  

作　　者：DING Jian CHEN Guo LI Jian-ming LI Kai-qi YANG Chen-yi WU Yue-rong 

机构地区：[1]Guangxi University of Tradit Chin Med,Nanning 530200,China [2]Department of Spleen and Stomach Diseases,Hubei Provincial Hospital of Tradit Chin Med,Wuhan 430060,China

出　　处：《Journal of Hainan Medical University》2023年第23期14-19,共6页海南医学院学报（英文版）

基　　金：National Natural Science Foundation Project(No.81860780);Guangxi Natural Science Foundation General Project(No.2020GXNSFAA297162)。

摘　　要：Objective:To investigate the effects of Compound Fufangteng Mixture(CFM)on the expression levels of CD11b^(+)monocytes surface activation molecules CD2,CD69 and depleted molecules PD-1 and CD95 in spleen and peripheral blood of immunosuppressed mice.Methods:30 healthy SPF Balb/c male mice were randomly divided into Control,immunosuppressive model group(CTX group),high,medium and low dose intervention group(CTX+CFM-H group,CTX+CFM-M group,CTX+CFM-L group),Astragalus particles(AP)intervention group(CTX+AP group).The mice in each intervention group were injected intraperitoneally with cyclophosphamide(CTX)70 mg/kg for 3 consecutive days,and then administered for 10 d according to the group regimen,and the materials were taken 24 h after the last dose.Flow cytometry detected the expression of CD11b^(+)monocytes surface activation molecules(CD2,CD69)and depleting molecules(PD-1,CD95)in mouse peripheral blood.Results:Compared with the control group,the expression levels of CD11b^(+)and CD69+in the spleen of CTX+CFM-H,CTX+CFM-M and CTX+CFM-L groups showed an improvement trend,and decreased with the dose gradient of CFM.Conclusion:CFM could regulate the immune function of splenic CD11b+ CD69 cells in immunosuppressed mice.

关 键 词：Compound fufangteng mixture IMMUNOSUPPRESSION CD69 CD95 

分 类 号：R285.5[医药卫生—中药学]