The mechanism of regulating macrophage polarization based on Notch1 signaling pathway to improve joint inflammation in adjuvant arthritis rats  

作　　者：CHENG Jing WAN Lei ZHAO Lei LI Shu LI Fang-ze HU Sai-sai CHEN Ying-ying 

机构地区：[1]Anhui University of Traditional Chinese Medicine,Hefei 230031,China [2]Department of Rheumatology,the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine,Hefei 230031,China

出　　处：《Journal of Hainan Medical University》2023年第23期20-25,共6页海南医学院学报（英文版）

基　　金：National Natural Science Foundation General Project(No.81973655,82274501);The Seventh Batch of National Elderly Traditional Chinese Medicine Experts'Academic Experience Inheritance Project of the National Administration of Traditional Chinese Medicine(No.Guo Zhong Yao Ren Jiao Han[2022]No.76);Anhui Provincial Excellent Youth Talent Support Program for Universities(No.Anhui Education Secretary[2022]No.11);Key Laboratory of Xin'an Medical Education Department(No.2020xayx04);Excellent Youth Talent Support Program for Universities(No.Anhui Education Secretary[2022]No.11);Provincial Education and Teaching Reform Research Project of Anhui Province Higher Education Quality Engineering Project(No.2022jyxm883)。

摘　　要：Objective:To study the impact of the Notch1/Jagged1/RBP-Jκ/Hes1 signaling pathway on macrophage polarization and its role in modulating the inflammatory response in rats with adjuvant arthritis(AA).Methods:The rats were randomly divided into three groups(6 rats):the healthy group(NC),the model group(MC),and the Notch1 inhibitor group(FLI).Medication was administered after 12 days of inducing inflammation.After 30 days,the arthritis index(AI)and degree of swelling in the right hind foot joint(E)were measured in each group.The expression levels of CD80^(+)and CD163^(+)cells in peripheral blood macrophages of rats were analyzed by flow cytometry.The standards of IL-4,IL-10,IL-1β,and TNF-α in rat serum were gauged by Enzyme-linked immunosorbent assay.The expression of Notch1,Jagged1,RBP-Jκ,and Hes1 proteins in rat synovial tissue was detected using Western blot.Results:The degree of swelling(E)and arthritis index(AI)in the MC group rats with AA were significantly higher than those in the NC group(P<0.01).CD80^(+)cell expression was significantly higher compared to the control group(P<0.01),while CD163^(+)cell expression was significantly lower than the control group(P<0.01).IL-1βand TNF-α expression levels were significantly elevated(P<0.01),whereas IL-4 and IL-10 expression levels were significantly decreased(P<0.01).Notch1,RBP-Jκ,Jagged1,and Hes1 protein expression levels were significantly increased(P<0.01).In comparison to the MC group,the rats in the Notch1 inhibitor group exhibited a significant reduction in toe swelling and arthritis index(P<0.01).CD80^(+)cell expression was significantly decreased(P<0.01),while CD163+cell expression was significantly increased(P<0.01).IL-1β and TNF-α expression levels were significantly decreased(P<0.05),whereas IL-4 and IL-10 levels were significantly increased(P<0.01).Notch1,Jagged1,Hes1,and RBP-Jκ protein expression levels were significantly decreased(P<0.05).Correlation analysis revealed a positive association between CD80^(+)and Notch1,Jagged1,Hes1,and RBP-Jκ(

关 键 词：Adjuvant arthritis Notch1/Jagged1/RBP-Jκ/Hes1 axis Macrophage polarization 

分 类 号：R285.5[医药卫生—中药学] R684.3[医药卫生—中医学]