Abnormal expression and significance of circ-CBLB/miR-486-5p in patients with rheumatoid arthritis of spleen deficiency and dampness excess type  

作　　者：LI Shu WAN Lei LIU Jian HUANG Chuan-bing CHEN Ying-ying LI Fang-ze HU Sai-sai CHENG Jing 

机构地区：[1]Anhui University of Traditional Chinese Medicine,Hefei 230031,China [2]Department of Rheumatology,the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine,Hefei 230031,China

出　　处：《Journal of Hainan Medical University》2023年第17期43-49,共7页海南医学院学报（英文版）

基　　金：the Seventh Batch of Inheritance Project of Academic Experience of National Veteran TCM Experts of the National Administration of Traditional Chinese Medicine (GZYYRJH[2022]No. 76);National Natural Science Foundation General Project (No.81973655);National Natural Science Foundation General Project (No.82274501);Anhui Provincial Excellent Youth Talent Support Program for Universities (Anhui Education Secretary [2022]No. 11);Key Laboratory of Xin'an Medical Education Department (No.2020xayx04);Natural Science Research Project of Universities in Anhui Province (No.KJ2020A0397);Anhui NewEra Education Quality Engineering Project (No.2022xscx103)。

摘　　要：Objective:To explore the abnormal expression and significance of circ-CBLB/miR-486-5p in patients with rheumatoid arthritis of spleen deficiency and dampness excess type.Methods:The 30 healthy individuals included in the method were from the Physical Examination Center of Anhui Provincial Hospital of Traditional Chinese Medicine,and the 60 rheumatoid arthritis patients included were from the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine.The disease activity score of patients with rheumatoid arthritis was evaluated using VAS score and DAS28 score,joint symptoms and spleen deficiency syndrome score were evaluated using graded quantification method,immune inflammation indicators were detected using relevant instruments,inflammatory cytokines were detected using ELISA method,macrophage markers were detected using FCM method,and pathway gene expression was detected using RT-qPCR;Evaluate the predictive effect of circ-CBLB and miR-486-5p on disease activity in rheumatoid arthritis using ROC curves.Results:(1)miR-486-5p,CD14^(+)CD86^(+),ESR,CRP,RF,Anti CCP Ab,IL-6,TNF in patients with rheumatoid arthritis-αThe levels of circ-CBLB,CD14^(+)CD163^(+),IL-4,and IL-10 were significantly higher than those of healthy individuals;(2)The expression level of circ-CBLB in patients with rheumatoid arthritis is positively correlated with CD14^(+)CD163^(+),and negatively correlated with miR-486-5p and CD14^(+)CD86^(+);The expression level of miR-486-5p is negatively correlated with CD14^(+)CD163^(+)and positively correlated with CD14^(+)CD86^(+);There is a negative correlation between CD14^(+)CD86^(+)and CD14^(+)CD163^(+);ESR is negatively correlated with circ-CBLB,and positively correlated with miR-486-5p,CD14^(+)CD86^(+),CRP;CRP is negatively correlated with circ-CBLB,CD14^(+)CD163^(+),and positively correlated with CD14^(+)CD86^(+),ESR;(3)The expression level of circ-CBLB in patients with rheumatoid arthritis is negatively correlated with joint tenderness,morning stiffness,lack of qi and lazy speech

关 键 词：Rheumatoid arthritis circ-CBLB MiR-486-5p Inflammatory polarization Spleen deficiency and dampness excess syndrome 

分 类 号：R259[医药卫生—中西医结合]