The expression of TUSC3 in Preeclampsia and the function in trophoblast cell  

作　　者：LI Meng-yong-wei KANG Jin-yu LIN Dan SUN Fei JIE Qiu-ling MA Yan-lin 

机构地区：[1]Hainan Provincial Key Laboratory for human reproductive medicine and Genetic Research,Hainan Provincial Clinical Research Center for Thalassemia,the Key Laboratory of Tropical Translational Medicine of Ministry of Education,Department of Reproductive Medicine,the First Affiliated Hospital of Hainan Medical University,Hainan Medical University,Haikou,Hainan 571101,China [2]Haikou Key Laboratory for Preservation of Human Genetic Resource,the First Affiliated Hospital of Hainan Medical University,Haikou,Hainan 571101,China

出　　处：《Journal of Hainan Medical University》2023年第18期30-35,共6页海南医学院学报（英文版）

基　　金：Hainan Natural Science Foundation project(822MS175);National Nature Science Foundation of China(No.82072880,82003144,81960283,82201874);Supported by the Specific Research Fund of the Innovation Platform for Academicians of Hainan Province;Project supported by Hainan Province Clinical Medical Center。

摘　　要：Objective:In this study,we aimed to explore the expression of TUSC3 in Preeclampsia and to research the potential function of TUSC3 in placental trophoblast cells.Methods:We collected 10 cases of normal placental tissues and preeclampsia placental tissues,respectively.These parturient received treatment at the First Affiliated Hospital of Hainan Medical University between June 1,2020,and December 31,2022.The expression of TUSC3 in placenta was detected by immunohistochemistry.The effect of TUSC3 on the migration and invasion of HTR8/SVneo cells was analyzed by migration assay and Transwell assay.Results:The expression of TUSC3 was slightly increased in placental villis in preeclampsia.Immunohistochemistry and qRT-PCR were used to detect the expression of TUSC3 protein and mRNA in placental tissues.TUSC3 was markedly upregulated in PE placental tissues(P<0.01).The results of migration assay and Transwell assay showed that the migration rate and the number of invasive cells were significantly decreased in HTR8 overexpressing TUSC3(P<0.01).Conclusions:TUSC3 was markedly increased in PE placental tissues and inhibited trophoblast cells migration and invasion.

关 键 词：PREECLAMPSIA TUSC3 Trophoblast cells Migration assay Transwell assay 

分 类 号：R714.244[医药卫生—妇产科学]