The effect and mechanism of stilbene glycosides on improving neuronal injury in Alzheimer's disease rats by regulating ASK/MKK7/JNK pathway  

作　　者：LI Yue KANG Bi-qian HE Xiao-xuan HU Rui XIAO Zhen LUO Chen-liang WU Gui-you HUANG Zhong-shi 

机构地区：[1]School of Basic Medicine,Youjiang Medical Univ for Nationalities,Baise 533000,China [2]School of Pharmacy,Guangxi Univ of Chinese Medicine,Baise 533000,China

出　　处：《Journal of Hainan Medical University》2023年第21期1-6,共6页海南医学院学报（英文版）

基　　金：National Natural Science Foundation of China (81860709);Project of Baise City Regional Multiple Development Joint Special Plan[Bai Zi (2022)41-39]。

摘　　要：Objective:To investigate the mechanism of action of tetrahydroxy stilbene glycosides(TSG)in ameliorating neuronal damage in Alzheimer's disease rats by regulating MKK7 and JNK kinases.Methods:A total of 24-month-old 42 SD rats were randomly selected for the experiment in 7 groups:normal group,sham-operated group,model group,positive drug group,low,medium and high dose TSG group at 0.033 g/kg,0.1 g/kg,0.3 g/kg.The Model Group and the TSG groups were established by stereotaxic Aβ25-35 solution.After 28 days,the model rats were selected by passive avoidance test.After screening,each dosage group of TSG and positive drug group was given intragastrically according to the corresponding dosage,and the experiment was carried out after 28 days.The pathological changes of hippocampal CA1 region were observed by tissue staining,and the amount of MKK7 and JNK proteins and the expression content of MKK7 and JNK mRNA by histochemical method of protein,and qRTPCR assay.Results:(1)He staining observation:Compared with the normal group and the sham-operated group,the number of nerve cells in the model group decreased and arranged irregularly,the cell membrane shrank,and the nucleus deformed and dissolved.The number of neurons in the positive drug group and TSG Group also increased significantly,the order is also relatively well.(2)From the results of the Tunel staining experiments:the positive apoptotic cells in the model group were higher than control group and sham-operated group,positive drug group and TSG drugs group was significantly smaller than that in the model group(P<0.05).(3)Compared with the control group and the Virtual Operation Group,the MKK7 and JNK protein concentrations in the brain of the model group were increased(P<0.05)by data analysis of immunohistochemistry:Compared with the model group,the protein expression of positive drug and TSG each dose group were reduced(P<0.05).(4)The results of QRTPCR data showed that the levels of MKK7 and JNK mRNA in the brain tissue of the model group were increased compared

关 键 词：Nervous system Alzheimer’s disease Stilbene glycoside MKK7 JNK 

分 类 号：R285.5[医药卫生—中药学] R749.16[医药卫生—中医学]