使用孕酮P4诱导雌鼠同步发情  

Estrus Synchronization Using Progesterone P4 in Female Rats and Mice

作　　者：李瑞娟 陈晓蕾 额尔敦达来 杨文东 郭铁钧 韩召 赵思凯 张海超 LI Ruijuan;CHEN Xiaolei;E ER DUN Dalai;YANG Wendong;GUO Tiejun;HAN Zhao;ZHAO Sikai;ZHANG Haichao(Biocytogen Pharmaceuticals(Beijing)Co.,Ltd.,Beijing 102609,China)

机构地区：[1]百奥赛图医药科技股份有限公司,北京102609

出　　处：《实验动物科学》2024年第1期39-44,共6页Laboratory Animal Science

摘　　要：目的 探索采用注射孕酮的方式诱导SD大鼠和BALB/c小鼠的雌鼠同步发情。方法 在实验第1天和第2天给8周龄SD大鼠和BALB/c小鼠的雌鼠注射孕酮激素和注射孕酮量分别为每只10 mg和2 mg,连续注射2 d,第4天合笼,分别在第5天、第6天和第7天进行检栓。利用见栓后的SD大鼠假孕鼠进行胚胎移植,选择发情雌鼠与结扎雄鼠交配得到的假孕鼠同步移植作为对照;利用见栓后的BALB/c小鼠雌鼠冲取囊胚用于胚胎干细胞(ESC)注射。结果 注射孕酮后的SD大鼠雌鼠单日最高见栓率可达到44%,整体见栓率能达到80%以上;注射孕酮后的BALB/c小鼠雌鼠单日最高见栓率可达到46%,整体见栓率能达到70%以上;注射孕酮单日见栓率是传统选择发情后合笼见栓的7~8倍,整体见栓率是传统方式的15倍以上,与传统方法相比,用于准备假孕的SD大鼠雌鼠和供体囊胚BALB/c小鼠雌鼠的基础雌鼠数量可降低10%~20%;SD大鼠假孕鼠的胚胎出生率较传统方法提高13%。结论 注射每只10 mg和每只2 mg孕酮可分别用于诱导8周龄SD大鼠雌鼠和BALB/c小鼠的雌鼠同步发情,可显著提高见栓率和SD大鼠雌鼠胚胎出生率。Objective To explore the induction of estrus synchronization in SD rats and BALB/c mice females by progesterone injection.Method Progesterone hormone was injected into 8 weeks SD rats and BALB/c mice females on the first and second day of the experiment at doses of 10 mg/rat and 2 mg/mouse respectively,and cohabited on the fourth day.The observations of copulatory plugs were performed on the fifth,sixth and seventh day of the experiment.Embryo transfer was performed in copulatory plug checked pseudopregnant SD rats,and pseudopregnant rats obtained by mating with vasoligated males were used as controls.Blastocysts were flushed from embolized BALB/c mice females for embryonic stem(ES)cell injection.Result The highest copulatory plug rate of SD rats females injected with progesterone reached 44%on a single day and more than 80%overall.The highest copulatory plug rate of BALB/c mice females injected with progesterone reached 46%on a single day and more than 70%overall.The copulatory plug rate of progesterone injected group was 7-8 times higher than traditional method group on a single day,and more than 15 times higher than traditional method group overall.Compared with the traditional method,in progesterone injected group,the colony population of SD rats females and BALB / c mice females could be reduced to 10% -20%;the embryo birth rate of SD rats pseudopregnant could increase by about 13%. Conclusion Progesterone injection 10 mg/ rat and 2 mg/ mouse can be used for estrus synchronization in 8 weeks SD rats and BALB / c mice females respectively, and can significantly increase the copulatory plug rate and SD rats embryo birth rate.

关 键 词：孕酮 胚胎移植 同步发情 SD BALB/C 假孕鼠 囊胚供体 

分 类 号：Q95-3[生物学—动物学]