Recent advances in the culture-independent discovery of natural products using metagenomic approaches  

作　　者：SHEN Yiping LIU Nan WANG Zongqiang 

机构地区：[1]Laboratory of Microbial Drug Discovery,China Pharmaceutical University,Nanjing 211198,China

出　　处：《Chinese Journal of Natural Medicines》2024年第2期100-111,共12页中国天然药物（英文版）

基　　金：supported by the Start-Up Grant from China Pharmaceutical University(No.3154070046).

摘　　要：Natural products derived from bacterial sources have long been pivotal in the discovery of drug leads.However,the cultivation of only about 1%of bacteria in laboratory settings has left a significant portion of biosynthetic diversity hidden within the genomes of uncultured bacteria.Advances in sequencing technologies now enable the exploration of genetic material from these metagenomes through culture-independent methods.This approach involves extracting genetic sequences from environmental DNA and applying a hybrid methodology that combines functional screening,sequence tag-based homology screening,and bioinformatic-assisted chemical synthesis.Through this process,numerous valuable natural products have been identified and synthesized from previously uncharted metagenomic territories.This paper provides an overview of the recent advancements in the utilization of culture-independent techniques for the discovery of novel biosynthetic gene clusters and bioactive small molecules within metagenomic libraries.

关 键 词：METAGENOMICS Culture-independent approach Natural product Functional screening Sequence tag-based homology screening Syn-BNP 

分 类 号：R284[医药卫生—中药学]