葡萄UBP基因家族对非生物胁迫的响应及VvUBP基因功能研究  

Response of UBP Gene Family to Abiotic Stress and Function of VvUBP Gene in Grape

作　　者：郭雁飞 徐涛[1] 陈天池 陈哲 杨杰 龚丽丽 斯昊璇 吴月燕[1] GUO Yanfei;XU Tao;CHEN Tianchi;CHEN Zhe;YANG Jie;GONG Lili;SI Haoxuan;WU Yueyan(College of Biology and Environment,Zhejiang Wanli University,Ningbo,Zhejiang 315100;College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306)

机构地区：[1]浙江万里学院生物与环境学院,浙江宁波315100 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《核农学报》2024年第1期33-45,共13页Journal of Nuclear Agricultural Sciences

基　　金：宁波市科技创新2025现代种业重大专项项目(2021Z005);浙江省重点研发计划(2021C02053);浙江省教育厅一般科研项目(Y202250321);浙江省“生物工程”一流学科学生创新计划项目(CX2022025)。

摘　　要：泛素特异蛋白酶是一个广泛存在于真核生物中且高度保守的基因家族,通过去泛素化作用调节细胞蛋白的各种生理活动。为了系统探究葡萄UBP基因家族功能,本研究运用生物信息学方法对葡萄UBP基因家族成员进行鉴定与分析,利用实时荧光定量PCR(qRT-PCR)技术分析VvUBPs基因在不同非生物胁迫下的表达模式。结果表明,VvUBP基因家族包括27个成员,编码序列(CDS)长度为594~4 812 bp,编码氨基酸序列长度为197~1 603 aa。系统进化分析结果显示,UBP基因家族可分为8个亚组(GⅠ~GⅧ)。VvUBPs在茎中的表达水平相对较低,VvUBP27、VvUBP10和VvUBP23分别在卷须、花和叶中表达水平较高。外源茉莉酸甲酯(MeJA)处理可显著诱导VvUBP5、VvUBP12、VvUBP18和VvUBP27等4个基因高表达,外源水杨酸(SA)处理可显著诱导VvUBP5和VvUBP19基因高表达。VvUBP15在盐胁迫浓度0.6%时的表达量增加最明显,VvUBP6在弱光胁迫下表达显著下降以及VvUBP25在高温胁迫6 h时增幅最大。综上,VvUBP基因家族成员能够响应不同的非生物胁迫,进而提高葡萄的抗逆性。本研究结果为揭示UBP调控葡萄生长发育和抗逆胁迫机理等提供了科学依据。Ubiquitin-specific proteases are a highly conserved gene family that is widely present in eukaryotes and regulate various physiological activities of cellular proteins through deubiquitination.In order to systematically explore the function of the UBP gene family in grape,this study used bioinformatics to identify and analyze the members of the UBP gene family in grape,and further assayed the expression patterns of VvUBP genes by quantitative real-time PCR under different abiotic stresses.The results showed that the VvUBP gene family included 27 members,with coding sequence(CDS)length between 594-4812 bp and coding amino acid sequence length of 197-1603 aa.The results of phylogenetic analysis showed that the UBP gene family could be divided into 8 subgroups(GⅠ-GⅧ).The expression levels of VvUBPs in stems were relatively low,and VvUBP27,VvUBP10 and VvUBP23 were expressed at higher levels in tendrils,flowers and leaves,respectively.Gene expression analysis showed that treatments with exogenous methyl jasmonate(MeJA)significantly induced high expression of four genes,including VvUBP5,VvUBP12,VvUBP18 and VvUBP27,and exogenous salicylic acid(SA)treatment significantly induced high expression of VvUBP5 and VvUBP19.The expression of VvUBP15 increased most significantly under 0.6%salt stress,the expression of VvUBP6 decreased significantly under low light stress and VvUBP25 increased the most at high temperature stress for 6 h.In summary,VvUBP gene family members were able to respond to different abiotic stresses,thereby improve the stress tolerance of grapes.The results of this study provide a scientific basis for revealing the mechanism of UBP regulating grape growth,development and stress resistance.

关 键 词：葡萄 UBP基因家族 生物信息学 表达分析 

分 类 号：S663.1[农业科学—果树学]