适于玉米杂交种复杂样品纯度鉴定的SSR复合检测方案的建立  

Identification of the Purity of Complex Samples by SSR Multiplexed Reaction System

作　　者：施昕晨 王蕊[1] 葛建镕[1] 张云龙 任洁[1] 王璐[1] 易红梅[1] 王凤格[1] SHI Xin-chen;WANG Rui;GE Jian-rong;ZHANG Yun-long;REN Jie;WANG Lu;YI Hong-mei;WANG Feng-ge(Maize Research Center,Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding,Beijing 100097,China)

机构地区：[1]北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室,北京100097

出　　处：《玉米科学》2024年第1期74-81,共8页Journal of Maize Sciences

摘　　要：为了解决玉米复杂样品纯度中存在遗传不稳定及回交株等难以鉴定的问题,通过分析3998份国家和省级审定玉米品种,结合快速提取、多重扩增体系等技术,构建一套适用于复杂样品纯度检测的复合检测方案。分析53份不同来源的郑单958纯度测试样品,结果表明,对于复杂样品使用单个位点进行纯度判定时,不同位点间结果会有较大差别,使用3个或3个以上位点综合判定时能够有效获得准确稳定的纯度检测结果。复合检测方案能够在时间和成本未大幅增加、仅增加少数引物的条件下获得多个SSR标记指纹,使复杂样品得到更加全面、精准解析,从而为种子生产中复杂样品的质量问题提供解决方案。To address the issue of difficult purity identification in complex maize samples,including genetic in⁃stability and backcross plants,a comprehensive detection scheme for purity detection in complex samples was devel⁃oped.This scheme was constructed by analyzing 3,998 national and provincial approved maize varieties,and incor⁃porating techniques such as rapid extraction and multiple amplification systems.By analyzing 53 samples of maize hybrid Zhengdan958 from different sources,it was found that when using a single locus for purity determination,there were significant differences in results among different loci due to genetic instability.However,when three or more loci were used for comprehensive determination,accurate and stable purity detection results could be ob⁃tained.The comprehensive detection scheme allows for the generation of multiple SSR marker fingerprints with mini⁃mal increase in time and cost,using only a few additional primers.This enables a more comprehensive and accurate analysis of complex samples,providing solutions for quality issues in seed production involving complex samples.

关 键 词：玉米 杂交种 复杂样品 纯度 SSR标记 复合检测方案 

分 类 号：S513.037[农业科学—作物学]