Ⅱ型登革病毒感染对白纹伊蚊生存影响的研究  

Research on the impact of type II dengue virus infection on the survival of Aedes albopictus

作　　者：庞兴亚 徐铁龙 郑彬 PANG Xingya;XU Tielong;ZHENG Bin(Anhui Provincial Center for Disease Control and Prevention,Hefei,Anhui 230601,China;Evidence-Based Medicine Research Center Department,Jiangxi University of Chinese Medicine,Nanchang,Jiangxi 330004,China;National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Chinese Center for Tropical Diseases Research,Key Laboratory on Parasite and Vector Biology of National Health Commission,WHO Collaborating Center for Tropical Diseases,National Center for International Research on Tropical Diseases,Ministry of Science and Technology,School of Global Health,Chinese Center for Tropical Diseases,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China)

机构地区：[1]安徽省疾病预防控制中心,安徽合肥230601 [2]江西中医药大学循证医学研究中心,江西南昌330004 [3]中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心),国家卫生健康委员会寄生虫病原与媒介生物学重点实验室,世界卫生组织热带病合作中心,科技部国家级热带病国际研究中心/上海交通大学医学院-国家热带病研究中心全球健康学院,上海200025

出　　处：《中国热带医学》2024年第2期196-199,共4页China Tropical Medicine

摘　　要：目的了解Ⅱ型登革病毒感染对白纹伊蚊生存的影响,为登革热防控提供基础资料。方法通过培养白纹伊蚊C6/36细胞,对Ⅱ型登革病毒进行扩增并收获。实验室驯化的白纹伊蚊羽化后,每天用现配的10%葡萄糖水喂养,于温度28℃,湿度≥70%条件下饲养,至3~5日龄时用于感染实验,感染前一天挑出雄蚊,将雌蚊分为感染组和未感染组,并断食断水。感染时分别吸食含与不含Ⅱ型登革病毒的血餐(未感染组血餐除不含Ⅱ型登革病毒外,其余成分与感染组血餐相同),挑取饱血蚊采用10%葡萄糖水维持饲养,每天观察、记录两组白纹伊蚊的生存情况。吸食血餐后第14天时,采用荧光定量PCR法检测感染组白纹伊蚊Ⅱ型登革病毒核酸,计算病毒感染率。计算并比较感染组与未感染组白纹伊蚊的生存率,用生存分析方法,绘制两组白纹伊蚊的生存曲线并进行log-rank检验。结果感染组共饲养99只感染实验后的饱血白纹伊蚊,未感染组共饲养83只未经感染实验饱血白纹伊蚊,感染实验后第14天,感染组和未感染组白纹伊蚊分别死亡31只和15只,死亡率分别为31.31%(31/99)和18.07%(15/83),感染组死亡率高于未感染组。经log-rank检验,感染组白纹伊蚊生存曲线低于未感染组(χ^(2)=4.1219,P<0.05)。荧光定量PCR检测感染组白纹伊蚊的感染率为77.94%(53/68)。结论Ⅱ型登革病毒感染白纹伊蚊可降低白蚊伊蚊生存率。Objective To investigate the effect of type II dengue virus infection on the survival rate of Aedes albopictus,to provide basic data for the prevention and control of dengue fever.Methods The Aedes albopictus C6/36 cells were cultured to amplify and harvest typeⅡdengue virus.After emergence,the lab-domesticated Aedes albopictus was fed daily with freshly prepared 10%glucose water under 28℃and humidity≥70%,and used for infection experiment at 3-5 days of age.The day before infection,male mosquitoes were selected out,and the female mosquitoes were divided into infected and uninfected groups,deprived of food and water.When infected,they respectively were fed blood meals containing or without dengue virus typeⅡ(the blood meal of the uninfected group was the same as the infected group's,minus the presence of TypeⅡdengue virus),and the saturated blood mosquitoes were fed with 10%glucose water.The survival of mosquitoes in two groups was observed and recorded daily.On the 14th day after blood feeding,the nucleic acid of dengue virus typeⅡof Aedes albopictus was detected by fluorescence quantitative PCR,and the infection rate was calculated.The survival rates of infected and uninfected Aedes albopictus were calculated and compared.The survival curves of the two groups were plotted using survival analysis and the log-rank test was performed.Results A total of 99 infected saturated blood Aedes albopictus were reared in the infected group,and 83 in the uninfected group.By the 14th day after infection,31 and 15 mosquitoes had died in the infected and uninfected groups respectively,with mortality rates of 31.31%(31/99)and 18.07%(15/83),respectively,

关 键 词：白纹伊蚊 登革病毒 生存分析 

分 类 号：R373.33[医药卫生—病原生物学]