球茎甘蓝qRT-PCR内参基因的筛选及稳定性验证  

Screening and stability verification of kohlrabi reference genes

作　　者：郭怡婷 孙世英 赵文菊 王鑫淼 李晓娟 马一栋 任延靖 GUO Yiting;SUN Shiying;ZHAO Wenju;WANG Xinmiao;LI Xiaojuan;MA Yidong;REN Yanjing(Qinghai University,Xining 810016,China;Qinghai University Academy of Agriculture and Forestry Sciences,Laboratory for Research and Utilization of Germplasm Resources in Qinghai Tibet Plateau,Xining 810016,China;State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学,青海西宁810016 [2]青海大学农林科学院,青藏高原种质资源研究与利用实验室,青海西宁810016 [3]青海大学,三江源生态和高原农牧业国家重点实验室,青海西宁810016

出　　处：《甘肃农业大学学报》2024年第1期144-152,共9页Journal of Gansu Agricultural University

基　　金：国家大宗蔬菜产业技术体系-西宁综合试验站项目(CARS-23-G26);青藏高原种质资源研究与利用实验室(2022-ZJ-Y01);青海省重点研发与转化项目(2021-NK-124);西宁市重大科技专项项目(2021-Z-03)。

摘　　要：【目的】通过对已知内参基因进行筛选,确定球茎甘蓝最合适的内参基因,确保实时荧光定量PCR(qRT-PCR)的准确表达。【方法】本试验选择了11种参考基因,利用GeNorm、Normalfinder和Bestkeep软件对紫色球茎甘蓝和绿色球茎甘蓝不同部位的内部参考基因进行了分析。【结果】3个分析结果显示,内参基因Tip41在球茎甘蓝不同组织部位表达最稳定。【结论】Tip41是作为内参基因的最佳选择,为球茎甘蓝后续的分子生物学相关研究提供基础。【Objective】By screening known reference genes,the most appropriate reference genes in cabbage were identified to ensure the accurate expression of quantitative real-time PCR(QRT-PCR).【Method】Eleven reference genes were selected in this experiment,and the internal reference genes of different parts of purple and green cabbage were analyzed by GeNorm,Normalfinder and Bestkeep software.【Result】Three analysis results showed that the internal reference gene Tip41 was expressed most stably in different tissue parts of kohlrabi.【Conclusion】Tip41 was the best choice as an internal reference gene,provide a basis for the subsequent molecular biology related studies of kohlrabi.

关 键 词：球茎甘蓝 内参基因 荧光定量 

分 类 号：Q943.2[生物学—植物学]