外源褪黑素对干旱胁迫下园林小菊生长及生理的影响  

Effects of exogenous melatonin on growth and physiology of garden chrysanthemum under drought stress

作　　者：罗艳 虎淘淘 高晓芬 赵钰磊 张黎[1] 严瑞 LUO Yan;HU Taotao;GAO Xiaofen;ZHAO Yulei;ZHANG Li;YAN Rui(College of Enology and Horticulture,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学葡萄酒与园艺学院,宁夏银川750021

出　　处：《甘肃农业大学学报》2024年第1期211-218,共8页Journal of Gansu Agricultural University

基　　金：宁夏回族自治区重点研发计划项目“彩菊切花种苗快繁及精细化栽培技术集成示范”(2022BBF03035)。

摘　　要：【目的】探究外源褪黑素(Melatonin,MT)对干旱胁迫下园林小菊的缓解作用及适宜浓度,为干旱和半干旱地区种植园林小菊提供理论支持和参考价值。【方法】以园林小菊绚秋凝红为试材,使用20%PEG作为干旱胁迫渗透剂,通过叶面喷施不同浓度MT(50、100、150、200μmol/L),研究MT对干旱胁迫下园林小菊幼苗生长及生理指标的影响。【结果】干旱胁迫条件下,外源喷施100μmol/L MT可有效缓解干旱胁迫对园林小菊幼苗造成的伤害。园林小菊幼苗叶绿素含量、叶片相对含水量较PEG处理分别增加37.4%、69.1%,相对电导率(REC)和丙二醛含量(MDA)分别降低55.7%和42.8%,过氧化氢(H_(2)O_(2))增加75.0%,脯氨酸(Pro)增加60.6%,超氧化物歧化酶(SOD)、过氧化物歧化酶(POD)、过氧化氢酶(CAT)活性分别是PEG处理的1.9倍、2倍、3.2倍。通过主成分分析结果可知,外源MT对园林小菊叶绿素含量、叶片相对含水量和地上部鲜质量产生较大影响。【结论】外源喷施100μmol/L MT能够有效缓解干旱胁迫对园林小菊绚秋凝红所造成的伤害,提高扦插幼苗的耐旱性。【Objective】The study aimed at exploring the alleviation effect of exogenous melatonin(MT)on garden chrysanthemum under drought stress and screening its suitable concentration,providing reference for planting garden chrysanthemum in arid and semi-arid areas.【Method】With the garden chrysanthemum Xuanqiu ninghong as the test material and the 20%PEG as a drought stress penetrant,the different concentrations of MT(50,100,150,200μmol/L)were sprayed on the leaves to study the its effects on the growth and physiological indexes of chrysanthemum seedlings under drought stress.【Result】Under the drought stress conditions,the exogenously spraying of 100μmol/L MT could effectively alleviate the damage caused by drought stress to the garden chrysanthemum seedlings.Compared with the PEG treatment,the chlorophyll content and relative water content of the leaves increased by 37.4%and 69.1%,respectively,and the relative conductivity(REC)and malonaldehyde content(MDA)decreased by 55.7%and 42.8%,respectively.Hydrogen peroxide(H_(2)O_(2))increased by 75.0%,and proline(Pro)by 60.6%.The activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)were 1.9,2,and 3.2 times of PEG treatment.The results of the Principal Component Analysis showed that exogenous MT mainly had a great influence on the chlorophyll content and relative water content of leaves,and on the fresh weight of shoots of garden chrysanthemum.【Conclusion】This study showed that the exogenously spraying of 100μmol/L MT could effectively alleviate the damage to the garden chrysanthemum Xuanqiu ninghong caused by drought stress,improving the drought tolerance of the cutting seedlings.

关 键 词：园林小菊 外源褪黑素 PEG干旱胁迫 生理指标 

分 类 号：S682.1[农业科学—观赏园艺]