甜菊苷生产固体废弃物中绿原酸类絮凝提取工艺研究  

Study on Flocculation Extraction of Chlorogenic Acid from Solid Waste of Stevioside Production

作　　者：孙允红 周华 王晓晴 李忠原 温柔 康怀兴[1] 侯林 SUN Yunhong;ZHOU Hua;WANG Xiaoqing;LI Zhongyuan;WEN Rou;KANG Huaixing;HOU Lin(Department of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Pharmacy Department 1,Yantai Penglai Hospital of Traditional Chinese Medicine,Yantai 265699,China;Audit Section,Yantai Penglai People's Hospital,Yantai 265600,China;Qingdao Academy of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Qingdao 266000,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]烟台市蓬莱中医医院药剂一科,山东烟台265699 [3]烟台市蓬莱人民医院审计科,山东烟台265600 [4]山东中医药大学青岛中医药科学院,山东青岛266000

出　　处：《现代中药研究与实践》2023年第6期68-73,共6页Research and Practice on Chinese Medicines

基　　金：山东省中医药科技项目(2020M009);国家中医药管理局高水平中医药重点学科-中药药剂学(国中医药人教函【2023】85号)。

摘　　要：目的优化甜菊苷生产的絮凝工艺,对絮凝废弃物中的绿原酸类成分进行提取工艺研究。方法以绿原酸、异绿原酸A转移率和莱鲍迪苷A保留率的综合评分为指标,综合考虑透光率,通过单因素考察确定最佳絮凝工艺。以绿原酸、异绿原酸A转移率为指标,通过正交实验设计,考察乙醇浓度、pH、溶剂量对提取工艺的影响。结果最佳絮凝工艺条件为:聚合氯化铝用量为5%,室温搅拌3min,无需静置,绿原酸、异绿原酸A转移率和莱鲍迪苷A保留率的综合评分为83.24%。最佳提取工艺条件为pH=1,8倍量60%乙醇提取绿原酸类成分,板泥中绿原酸、异绿原酸A百分含量分别为0.96%、3.29%。结论优选的絮凝提取工艺提取率高、重复性好,适用于甜菊苷生产固体废弃物中绿原酸类成分的提取。Objective To optimize the flocculation process of stevioside production and study the extraction process of chlorogenic acids from the flocculated waste board mud.Methods The transfer rate of chlorogenic acid,isochlorogenic acid A and the retention rate of rebaudioside A were taken as the index,the light transmittance was considered and the optimal flocculation process was determined by single factor.With the transfer rate of chlorogenic acid and isochlorogenic acid A as index,the effects of ethanol concentration,pH and solvent on the extraction process were investigated through orthogonal experiment design.Results The optimal flocculation conditions were as follows:the dosage of polyaluminum chloride was 5%,stirring at room temperature for 3 min without standing,the comprehensive score of transfer rate of chlorogenic acid,isochlorogenic acid A and retention rate of rebaudioside A were 83.24%.The optimal extraction conditions were as follows:when pH was 1,8 times the amount of 60%ethanol extracted chlorogenic acid,chlorogenic acid and isochlorogenic acid A contents were 0.96%and 3.29%,respectively.Conclusion The optimized flocculation extraction technology has high extraction rate and good repeatability,and its suitable for the extraction of chlorogenic acids from solid waste of stevioside production.

关 键 词：甜叶菊 絮凝 提取工艺 绿原酸 异绿原酸A 

分 类 号：R283.3[医药卫生—中药学]