宁南霉素对水稻纹枯病的防治试验  

Preventionandcontrolof ricesheathblig twithningnanmycin

作　　者：宋春华 马骞 苏佳萌 邱晓杰 常缨[1] SONG Chunhua;MA Qian;SU Jiameng;QIU Xiaojie;CHANG Ying(School of Life Science,Northeast Agricultural University,Haerbin 150036,China)

机构地区：[1]东北农业大学生命科学学院,哈尔滨150036

出　　处：《农药》2024年第3期217-223,共7页Agrochemicals

基　　金：国家自然科学基金(32270394)。

摘　　要：[目的]水稻纹枯病是水稻主要病害之一,严重影响水稻的产量。宁南霉素(ningnanmycin)可有效防治多种农作物病害。[方法]测定了在感染纹枯病的水稻上喷施宁南霉素的生理生化指标;通过qRT-PCR分析了病程相关蛋白相关基因PR1-α、PR-3和PR-5的表达。[结果]与Rhizoctonia solani组相比,ningnanmycin+Rhizoctonia solani组水稻叶片的叶绿素a、叶绿素b、类胡萝卜素含量和β-1,3-葡聚糖酶、内切几丁质酶、PPO的活性在4~7 d显著增加;MDA、O_(2)^(-)和H_(2)O_(2)的含量在3~7 d显著降低;PAL、SOD、POD和CAT的活性在3~7 d显著增加;ningnanmycin+Rhizoctonia solani组水稻叶片的OsPR1-α、OsPR-3和OsPR-5基因的相对表达量在3~7 d显著高于Rhizoctonia solani组。[结论]宁南霉素通过诱导产生病程相关蛋白和保护酶,增强植株寄主抗性,可有效控制立枯丝核菌引起的水稻纹枯病。[Aims] Rice(Oryza sativa L.) sheath blight is one of the major diseases of rice, seriously harming rice yield. Ningnanmycin can effectively prevent and control a variety of crop diseases. [Methods] This study measured the physiological and biochemical indicators on rice infected with Rhizoctonia solani after spraying ningnanmycin;the expression of disease course-related protein-related genes PR1-α, PR-3 and PR-5 was analyzed by qRT-PCR.[Results] Compared with the Rhizoctonia solani group, the contents of chlorophyll a, chlorophyll b, total carotenoid and the activities of β-1,3-glucanase, endochitinase and PPO in rice leaves of the ningnanmycin+R. solani group were significantly higher on day 4 to 7;the content of MDA, O_(2)^(-), and H_(2)O_(2) significantly decreased from day 3 to day 7;the activities of PAL, SOD, POD, and CAT significantly increased from day 3 to day 7;the relative expression of OsPR1-α,OsPR-3 and OsPR-5 in rice leaves of the ningnanmycin+R. solani group were significantly higher than those of the R. solani group from day 3 to day 7. [Conclusions] Ningnanmycin can effectively control rice sheath blight caused by R. solani by inducing the production of disease-related proteins and protective enzymes to enhance plant host resistance.

关 键 词：水稻 宁南霉素 水稻纹枯病 立枯丝核菌 

分 类 号：S482.2[农业科学—农药学]