反复呼吸道感染患儿外周血单个核细胞中TLR2、TLR4表达情况与Th1/Th2免疫应答的关系  

The relationship between the expression of TLR2 and TLR4 in peripheral blood mononuclear cells and Th1/Th2 immune response in children with recurrent respiratory tract infection

作　　者：赵淑景[1] 马志平 张金彪[2] 付峰 冯娜娜[3] ZHAO Shujing;MA Zhiping;ZHANG Jinbiao;FU Feng;FENG Nana(Department of Pediatrics,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine,Cangzhou,Hebei 061000,China;Department of Laboratory Diagnosis,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine,Cangzhou,Hebei 061000,China;Department of Science and Education,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine,Cangzhou,Hebei 061000,China)

机构地区：[1]沧州中西医结合医院儿科一区,河北沧州061000 [2]沧州中西医结合医院实验诊断科,河北沧州061000 [3]沧州中西医结合医院科教科,河北沧州061000

出　　处：《国际检验医学杂志》2024年第6期663-666,共4页International Journal of Laboratory Medicine

基　　金：河北省医学科学研究课题计划项目(20200606)。

摘　　要：目的探讨反复呼吸道感染(RRTI)患儿外周血单个核细胞(PBMC)Toll样受体2(TLR2)、Toll样受体4(TLR4)表达情况与辅助性T细胞1(Th1)/辅助性T细胞2(Th2)免疫应答的关系。方法将2020年12月至2022年12月该院收治的65例确诊为RRTI的患儿纳入研究作为RRTI组。另选取同期于该院体检的健康儿童45例作为对照组。采用实时荧光定量PCR(qPCR)检测PBMC中TLR2和TLR4的信使核糖核酸(mRNA)相对表达水平。采用流式细胞仪检测PBMC中TLR2和TLR4蛋白表达率。采用酶联免疫吸附法(ELISA)检测血浆中Th1细胞因子干扰素-γ(IFN-γ)、Th2细胞因子白细胞介素-4(IL-4)水平及二者比值(IFN-γ/IL-4)。采用Pearson相关分析TLR2、TLR4蛋白表达率与血浆IFN-γ、IL-4水平的相关性。结果RRTI组血浆Th2细胞因子IL-4水平高于对照组,血浆Th1细胞因子IFN-γ水平低于对照组,IFN-γ/IL-4低于对照组,差异均有统计学意义(P<0.05)。RRTI组PBMC中TLR2和TLR4 mRNA相对表达水平及蛋白表达率均高于对照组,差异均有统计学意义(P<0.05)。Pearson相关分析显示,RRTI患儿PBMC中TLR2和TLR4蛋白表达率与血浆IFN-γ水平及IFN-γ/IL-4均呈负相关(P<0.05),与血浆IL-4水平均呈正相关(P<0.05)。结论RRTI患儿PBMC中TLR2、TLR4的表达及血浆Th1/Th2细胞因子均可能参与疾病的发生、发展的过程。过度活化的TLR2和TLR4可能会使Th1功能减弱、Th2功能增强。Objective To investigate the expression of Toll-like receptor 2(TLR2)and Toll-like receptor 4(TLR4)in peripheral blood mononuclear cells(PBMC)in children with recurrent respiratory tract infection(RRTI)and its relationship with T helper cell 1(Th1)/T helper cell 2(Th2)immune response.Methods A total of 65 children diagnosed with RRTI who admitted to the hospital from December 2020 to December 2022 were enrolled in the study as the RRTI group,and 45 healthy children who underwent physical examination in the hospital during the same period were enrolled as the control group.The relative expression levels of TLR2 and TLR4 mRNA in PBMCs were detected by real-time fluorescence quantitative PCR(qPCR).The expression rates of TLR2 and TLR4 protein in PBMCs were detected by flow cytometry.The levels of Th1 cytokine interferon-γ(IFN-γ),Th2 cytokine interleukin-4(IL-4)and their ratio(IFN-γ/IL-4)in plasma were detected by enzyme-linked immunosorbent assay(ELISA).Pearson correlation analysis was used to analyze the correlation between TLR2,TLR4 protein expression rates and plasma IFN-γ,IL-4 levels.Results The RRTI group had significantly higher plasma level of Th2 cytokine IL-4 than the control group,significantly lower plasma level of Th1 cytokine IFN-γthan the control group,and significantly lower ratio of IFN-γ/IL-4 than the control group,the differences were all statistically significant(P<0.05).The relative expression levels of TLR2 and TLR4 mRNA and protein expression rates in PBMC of children in the RRTI group were higher than those in the control group,and the differences were statistically significant(P<0.05).Pearson correlation analysis showed that the protein expression rates of TLR2 and TLR4 in PBMC of children with RRTI were both negatively correlated with both plasma IFN-γlevels and IFN-γ/IL-4(P<0.05)and positively correlated with plasma IL-4 levels(P<0.05).Conclusion The expression of TLR2 and TLR4 in PBMC and plasma Th1/Th2 cytokines in children with RRTI may be involved in the occurrence and developme

关 键 词：反复呼吸道感染 TOLL样受体 辅助性T细胞 免疫应答 儿童 

分 类 号：R446.63[医药卫生—诊断学]