不同质量浓度Cu^(2+)和Ag^(+)对大麦成熟胚组培特性的影响  

Effects of Different Mass Concentrations of Cu^(2+)and Ag^(+)on the Tissue Culture Characteristics of Barley Mature Embryos

作　　者：周洪斌 王卫斌[1] 王梦玥 熊静蕾 陈升位[1] 毛孝强[1] ZHOU Hongbin;WANG Weibin;WANG Mengyue;XIONG Jinglei;CHEN Shengwei;MAO Xiaoqiang(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2024年第1期9-17,共9页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(32060457);云南省科技厅基础研究重点项目(202201AS070066)。

摘　　要：【目的】探究不同质量浓度Cu^(2+)和Ag^(+)对大麦成熟胚组培特性的影响。【方法】以Bowman、北青7号和光头大麦的成熟胚为材料,研究不同质量浓度Cu^(2+)和Ag^(+)对大麦成熟胚愈伤诱导率、长根率、长芽率、褐化率、绿化率、再分化率和愈伤湿质量的影响。【结果】Bowman、北青7号和光头大麦成熟胚的7种组培特性间存在不同程度差异。与对照相比,0.75 mg/L Cu^(2+)可减少10.43%的成熟胚长根、7.48%的成熟胚长芽、8.24%的愈伤组织褐化、20.74 mg的愈伤湿质量;4.00 mg/L Ag^(+)可提高6.47%的愈伤组织诱导率,可减少9.08%的成熟胚长根、7.96%的成熟胚长芽和9.67%的愈伤褐化;0.75 mg/L Cu^(2+)和4.00 mg/L Ag^(+)对愈伤细胞转绿、愈伤增殖和再分化均无抑制。【结论】适宜质量浓度的Cu^(2+)和Ag^(+)可有效抑制大麦成熟胚长根、长芽和愈伤组织褐化,Ag+还可有效提高愈伤诱导率;在基于MS培养基的大麦成熟胚愈伤组织诱导和再分化培养中,Cu^(2+)和Ag^(+)的适宜质量浓度分别为0.75和4.00 mg/L。研究结果可为构建大麦成熟胚的愈伤诱导及其再分化技术体系提供理论支持。[Purpose]To investigate the effects of different mass concentrations of Cu^(2+)and Ag^(+)on the tissue culture characteristics of barley mature embryos.[Methods]Three barley genotypes,including Guangtoudamai,Beiqing No.7 and Bowman,were used to evaluated the effects of different mass concentrations of Cu^(2+)and Ag^(+)on the callus induction rate,rooting rate,shooting rate,browning rate,green-dot rate,redifferentiation rate and wet weight of callus of the barley mature embryos.[Results]The seven tissue culture characteristics were different among the mature embryos of Bowman,Beiqing No.7 and Guangtoudamai.Compared with the control,the rooting rate was reduced with 10.43%,shooting rate was reduced with 7.48%,browning rate was reduced with 8.24%,and wet weight of callus reduced with 20.74 mg,under 0.75 mg/L Cu^(2+).Under 4.00 mg/L Ag^(+),the callus induction rate was increased with 6.47%,rooting rate was reduced with 9.08%,shooting rate was reduced with 7.96%,and browning rate was reduced with 9.67%.The 0.75 mg/L Cu^(2+)and 4.00 mg/L Ag^(+)treatment did not showed any inhibition for the green-dot rate,redifferentiation rate and wet weight of per unit callus.[Conclusion]Cu^(2+)and Ag^(+)with appropriate mass concentration can effectively inhibit the rooting,shooting of barley mature embryo and callus browning,and Ag^(+)can also effectively improve the callus induction rate;the appropriate mass concentrations of Cu^(2+)and Ag^(+)in MS medium used to induce barley mature embryo callus and redifferentiate the calluses are 0.75 and 4.00 mg/L,respectively.The results can provide theoretical support for the construction of a callus induction and redifferentiation technology system for barley mature embryo.

关 键 词：大麦 成熟胚 组培特性 Cu^(2+)质量浓度 Ag^(+)质量浓度 

分 类 号：S512.301[农业科学—作物学]