过敏性紫癜病证结合实验动物模型研究进展  

Research Progress of Henoch-Schonlein Purpura Experimental Animal Model Based on the Combination of Disease and Syndrome

作　　者：郑茜 任献青[1,2] Zheng Qian;Ren Xianqing(School of Pediatrics,Henan University of Chinese Medicine,Zhengzhou 450000,China;The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,China)

机构地区：[1]河南中医药大学儿科医学院,郑州450000 [2]河南中医药大学第一附属医院,郑州450000

出　　处：《世界科学技术-中医药现代化》2023年第12期3955-3960,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委员会联合基金项目(U2004107):基于IgA1糖基化异常及Th17/Treg细胞失衡的祛风消癜方治疗过敏性紫癜的有效机制研究,负责人:任献青;河南中医药大学学科建设办公室河南省特色骨干学科中医学学科建设项目基础研究重点项目(STG-ZYXKY-2020021):基于调控PI3K/Akt/NOS通路和IgA1糖基化异常的凉血退紫方治疗过敏性紫癜血热妄行证的作用机制研究,负责人:任献青;河南中医药大学2021年度研究生科研创新类项目一般项目(2021KYCX046):基于Th1/Th2细胞和一氧化氮(NO)失衡的凉血退紫方治疗儿童HSP的有效机制研究,负责人:郑茜。

摘　　要：过敏性紫癜(Henoch-Schonlein purpura,HSP)病证结合实验动物模型法造模时间较短、操作性强、可重复率高,更适用于中医、中西医结合研究。但目前尚存在以下不足:①缺乏模型评价标准。动物模型建立成功的标准应依据何种标准,以后应与中兽医合作,以动物特有的信息结合系统生物学的理论和方法,综合基因组、蛋白质组和代谢组等各个层面开展组学研究,并通过数据的整合,制定HSP动物模型的判定标准。证候方面:现有的模型均为采用热性药物灌胃的方式造成动物瘀热证模型,但证候是否成功建立无判定标准,以后可在单纯热性药物灌胃的基础上联合乙醇、高脂饮食、脂肪乳等,并对不同联合组进行比较。证候评价方面引入四诊量化辨证体系,建立过敏性紫癜中医瘀热证候模型评价量表。②动物模型与临床实际存在差异。目前的病证结合模型集中于紫癜性肾炎,而在单纯皮肤型过敏性紫癜的动物模型构建上,动物模型的皮肤损伤(如紫癜、紫斑)率较低,以后可借鉴其他过敏性疾病的造模方法,在操作步骤中增加激发次数,以提高皮肤损伤率。③现有的HSP模型均为先造瘀热证模型,再造HSP疾病模型,但实际的疾病发生是病中有证,且证是疾病发展过程中某一阶段的病理概括,是动态变化的,以后应同时在实验动物上进行病因与证因的干预操作,以搭建出病证结合的稳定时间窗口。Henoch-Schonlein purpura(HSP)combination of disease and syndrome model has short modeling time,strong operability and high repeatability,which is more suitable for the research of TCM and integrated traditional Chinese and Western medicine.However,there are still the following deficiencies:①Lack of model evaluation criteria.What criteria should be used to establish successful animal models and whether the diagnostic criteria for human HSP are of reference significance?In the future,we should cooperate with Chinese veterinary medicine to carry out omics studies at various levels including genome,proteome and metabolome by combining the unique information of animals with the theories and methods of systems biology,and formulate the criteria for determining HSP animal models through data integration.In terms of syndrome:all the existing models are the model of animal stasis and heat syndrome caused by gavage of heat-induced drugs,but there is no criterion for the successful establishment of syndrome.In the future,it can be combined with ethanol,high-fat diet and fatty milk on the basis of simple heat-induced drug gavage,and compare different combination groups.In the aspect of syndrome evaluation,the four-diagnosis quantitative syndrome differentiation system was introduced to establish the TCM syndrome model evaluation scale of Henoch-Schonlein Purpura′s stasis and heat.②There are great differences between animal model and clinical practice,and the reproducibility is low.At present,the combined models of disease and syndrome focus on purpura nephritis,and in the establishment of the animal model of pure dermal allergic purpura,the skin damage rate of the animal model(such as purpura,purpura)is low.In the future,we can learn from the modeling method of other allergic diseases,and increase the excitation times in the operation steps,in order to improve the skin damage rate.③All the existing HSP models are the syndrome model of blood stasis and heat first,and the disease model of HSP is reconstructed.However,t

关 键 词：过敏性紫癜 动物模型 病证结合 瘀热证 

分 类 号：S852.55[农业科学—基础兽医学]