基于黄独素B含量分析的甘草、当归炮制黄药子减毒工艺研究  

Study on the detoxification technology of Glycyrrhiza uralensis and Angelica sinensis pro⁃cessed-Dioscoreae Bulbiferae based on the content difference analysis of diosbulbin B

作　　者：宋玲玲 王君明[1,2,3] 张月月 刘鸣昊[4] 李炳印[1] 李亚敏 段雅倩 SONG Ling-ling;WANG Jun-ming;ZHANG Yue-yue;LIU Ming-hao;LI Bing-yin;LI Ya-min;DUAN Ya-qian(College of Pharmacy,Henan University of Chinese Medicine,Zhengzhou,450046,China;Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment&Chinese Medicine Development of Henan Province,Henan University of Chinese Medicine,Zhengzhou,450046,China;Collaborative Innovation Cen-ter of Research and Development on the Whole Industry Chain of Yu-yao,Zhengzhou 450046,China;.First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450006,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南中医药大学呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046 [3]豫药全产业链研发河南省协同创新中心,河南郑州450046 [4]河南中医药大学第一附属医院,河南郑州450000

出　　处：《时珍国医国药》2023年第12期2933-2936,共4页Lishizhen Medicine and Materia Medica Research

基　　金：河南省重点研发与推广专项(182102310255)。

摘　　要：目的基于黄独素B(DB)含量差异优选黄药子炮制减毒工艺。方法以炮制过程中常见的主药与辅料(主药:黄药子;辅料:甘草、当归)配比、炮制温度、炮制时间为考察因素,分别制备黄药子各炮制品。以乙腈:水为25∶75为流动相,柱温30℃,建立黄药子的高效液相色谱方法,进行等度洗脱,测定黄药子中主要毒性成分DB的含量。结果(1)与黄药子生品比较:甘草药汁制黄药子各炮制品中DB含量均有所下降,其中以A_(3)B_(1)C_(3)组中DB含量下降的最多,为0.781 mg/g,逆转率为31.9%;(2)当归药汁制黄药子各炮制品中DB含量均有所下降,其中以A_(3)B_(2)C_(1)组中DB含量下降的最多,为0.811 mg/g,逆转率为29.3%。结论甘草药汁制黄药子的最佳炮制减毒工艺为A_(3)B_(1)C_(3)组,即“黄药子∶甘草以100∶20的情况下,120℃炮制20 min”;当归药汁制黄药子的最佳炮制减毒工艺为A_(3)B_(2)C_(1)组,即“黄药子∶当归以100∶20的情况下,140℃炮制10 min”。Objective To optimize the detoxification technology of Dioscoreae Bulbiferae based on the content difference analysis of diosbulbin B(DB)by herbal-processing.Methods Taking the key factors in the processing process,such as the ratio of main drug to adjuvant(main drug:Dioscoreae Bulbiferae;adjuvant:Glycyrrhiza uralensis,Angelica sinensis),processing temper⁃ature,processing time,etc.,as the investigation factors to prepare the processed products of Dioscoreae Bulbiferae by herbal-processing.A high-performance liquid chromatography method was established with acetonitrile:water(25:75)as mobile phase and column temperature of 30℃,and isocratic elution was carried out to determine the content of toxic component of diosbulbin B(DB)in Dioscoreae Bulbiferae.Results Compared with Dioscoreae Bulbiferae group:(1)The content of DB in the processed products of Glycyrrhiza uralensis processed Dioscoreae Bulbiferae decreased to a certain extent,and the content of DB in A_(3)B_(1)C_(3)group decreased the most,which was 0.781 mg/g,and the reversal rate was 31.9%.(2)The content of DB in the processed products of Angelica sinensis processed Dioscoreae Bulbiferae decreased to a certain extent,and the content of DB in A3 B2 C1 group decreased the most,which was 0.811 mg/g,and the reversal rate was 29.3%.Conclusion The optimal detoxification technology of Glycyrrhiza uralensis processed Dioscoreae Bulbiferae was A_(3)B_(1)C_(3)group,that is,“when the ratio of Dioscoreae Bul⁃biferae to Glycyrrhiza uralensis was 100:20,processed at 120℃for 20 min”.The best detoxification technology of Angelica sinensis processed Dioscoreae Bulbiferae was A3 B2 C1 group,that is,“when the ratio of Dioscoreae Bulbiferae to Angelica sinensis was 100∶20,processed at 140℃for 10 min”.

关 键 词：黄药子 炮制减毒 甘草药汁制黄药子 当归药汁制黄药子 黄独素B 

分 类 号：R283[医药卫生—中药学]