醛化鹅红细胞用于百日咳菌液血凝滴度测定的可行性研究  

Feasibility study of hydroformylated goose red blood cells in the determination of hemagglutination titers of Bordetella pertussis fluid

作　　者：杨坤 谭大权 杨勇[1] 吴永超[1] 闵宪捷 李世玲 黄丹 雍雪飞[1] 蒲俊楠 张飞伟[1] Yang Kun;Tan Daquan;Yang Yong;Wu Yongchao;Min Xianjie;Li Shiling;Huang Dan;Yong Xuefei;Pu Junnan;Zhang Feiwei(Bacterial Vaccine Department 1,Chengdu Institute of Biological Products Co.,Ltd.,Chengdu 610023,China)

机构地区：[1]成都生物制品研究所有限责任公司细菌性疫苗一室,成都610023

出　　处：《国际生物制品学杂志》2024年第1期15-18,共4页International Journal of Biologicals

摘　　要：目的初步评价醛化鹅红细胞用于百日咳菌液血凝滴度测定的可行性,并确定其使用有效期.方法以新鲜鹅红细胞为对照,筛选出测定百日咳菌液血凝滴度的最佳醛化鹅红细胞浓度.用3批醛化鹅红细胞分别检测3批百日咳菌液血凝滴度,考察与新鲜鹅红细胞检测结果的一致性.观察不同放置期的醛化鹅红细胞发生溶血的情况,并考察血凝滴度检测结果与新鲜鹅红细胞的一致性.结果用于百日咳菌液血凝滴度测定的最佳醛化鹅红细胞浓度为0.33%.不同批次醛化鹅红细胞检测百日咳菌液血凝滴度的结果均与新鲜鹅红细胞检测结果一致.醛化鹅红细胞在2~8℃存放90 d仍能准确检测百日咳菌液血凝滴度,120 d后发生溶血现象.结论0.33%的醛化鹅红细胞可用于百日咳菌液血凝滴度测定,醛化鹅红细胞使用有效期为90 d.Objective To primarily evaluate the feasibility of using hydroformylated goose red blood cells(RBCs)in the determination of hemagglutination(HA)titers of Bordetella pertussis fluid,and to determine the period of validity.Methods The optimal working concentration of hydroformylated goose RBCs in HA test was screened using fresh goose RBCs as control.HA titers of 3 batches of Bordetella pertussis fluid were tested by 3 batches of hydroformylated goose RBCs respectively,and results were compared with those of fresh goose RBCs.The hemolysis of hydroformylated goose RBCs stored for different periods was observed and HA test results were compared with those of fresh goose RBCs.Results The optimal concentration of hydroformylated goose RBCs in the HA test of Bordetella pertussis fluid was 0.33%.HA titers of Bordetella pertussis fluid measured with different batches of hydroformylated goose RBCs were consistent with the results by fresh goose RBCs.HA titers of Bordetella pertussis fluid were still accurately detected using hydroformylated goose RBCs stored at 2-8°C for 90 d,but hemolysis occurred after 120 d.Conclusion The 0.33%hydroformylated goose RBCs are feasible for HA test of Bordetella pertussis fluid and the period of validity is 90 d.

关 键 词：博德特菌 百日咳 血凝集试验 溶血 醛化鹅红细胞 

分 类 号：R392-33[医药卫生—免疫学]