复方西羚解毒胶囊调控JAK2-STAT3信号通路抗H1N1病毒感染作用  

Anti-H1N1 effect of Compound Xiling Jiedu Capsules via the JAK2-STAT3 signaling pathway

作　　者：赵方舒 孟兆青 曹桂云 倪雯婷 田景振[1] 侯林 ZHAO Fangshu;MENG Zhaoqing;CAO Guiyun;NI Wenting;TIAN Jingzhen;HOU Lin(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong Hongjitang Pharmaceutical Group Co.,LTD,Jinan 250103,China;Qingdao Academy of Chinese Medical Sciences,Shandong University of Traditional Chinese Medicine,Qingdao 266112,China)

机构地区：[1]山东中医药大学药学院,济南250355 [2]山东宏济堂制药集团股份有限公司,济南250103 [3]山东中医药大学青岛中医药科学院,青岛266112

出　　处：《中华中医药杂志》2024年第2期941-946,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：山东省重点研发计划(重大科技创新工程)项目(No.2020CXGC010505,No.2021CXGC010511);济南市“新高校20条”资助项目(No.2021GXRC028)。

摘　　要：目的:研究复方西羚解毒胶囊抗H1N1病毒感染作用,并探讨其初步作用机制。方法:建模给药后,解剖,测定小鼠肺指数,肺病毒载量,肺组织病理变化,血清中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)细胞因子分泌水平,肺泡巨噬细胞分型及眼球血CD4^(+)/CD8^(+)T细胞比例,检测JAK2-STAT3信号通路相关基因mRNA和蛋白表达。结果:与模型组比较,复方西羚解毒胶囊高、中剂量组可显著降低小鼠肺指数(P<0.01),抑制H1N1病毒的复制载量(P<0.01,P<0.05),减轻肺组织病理状况,显著降低TNF-α、IL-1β、IL-6的分泌(P<0.05,P<0.01),显著升高肺泡巨噬细胞M2抗炎表型的比例(P<0.01),降低外周血CD4^(+)/CD8^(+)T细胞比例(P<0.01),降低IFN-β、JAK2、STAT3、OAS1 mRNA表达(P<0.01,P<0.05),降低p-JAK2/JAK2、p-STAT3/STAT3蛋白表达(P<0.01)。结论:复方西羚解毒胶囊对H1N1病毒感染具有显著的治疗效果,这可能是通过调节JAK2-STAT3信号通路发挥炎性控制和免疫调节作用实现的。Objective:To study the anti-H1N1 virus effect of compound Xiling Jiedu Capsules and its primary mechanism.Methods:After modeling and administration,the lung index,lung viral load,pathological changes of lung tissue,serum levels of TNF-α,IL-1β,IL-6 cytokine secretion,alveolar macrophage typing and the proportion of CD4^(+)/CD8^(+)T cells in eye blood were determined by dissection.mRNA and protein expression of genes related to JAK2-STAT3 signaling pathway were detected.Results:Compared with the model group,compound Xiling Jiadu Capsules medium-and high-dose groups could reduce lung index(P<0.01),inhibit the replication of H1N1 virus(P<0.01,P<0.05),alleviate the pathological condition of lung tissue,reduce the secretion of TNF-α,IL-1βand IL-6(P<0.05,P<0.01),significantly increase the proportion of anti-inflammatory phenotype M2 in alveolar macrophages(P<0.01),suppress the proportion of CD4^(+)/CD8^(+)T cells in peripheral blood(P<0.01),decrease the mRNA expression of IFN-β,JAK2,STAT3 and OAS1(P<0.01,P<0.05),and inhibit the protein expression of p-JAK2/JAK2 and p-STAT3/STAT3(P<0.01).Conclusion:Compound Xiling Jiedu Capsules has significant therapeutic effect on H1N1 virus infection,which may be achieved by regulating JAK2-STAT3 signaling pathway to exert inflammatory control and immune regulation.

关 键 词：复方西羚解毒胶囊 H1N1病毒 CD4+/CD8+T细胞比例 肺泡巨噬细胞 JAK2-STAT3信号通路 机制 

分 类 号：R285.5[医药卫生—中药学]