功能化聚联乙炔囊泡比色法检测Ⅰ型胶原蛋白  

Functionalized Polyacetylene Vesicles Colorimetric Assay for the Detection of Type Ⅰ Collagen

作　　者：郭纪彤 尹越 王宁馨 罗振宇 蒋波[1] GUO Jitong;YIN Yue;WANG Ningxin;LUO Zhenyu;JIANG Bo(Biomaterials Engineering Research Center,Sichuan University,Chengdu 610065,China)

机构地区：[1]四川大学生物材料工程研究中心,四川成都610065

出　　处：《生物化工》2024年第1期47-53,共7页Biological Chemical Engineering

摘　　要：目的:构建一种功能化聚联乙炔囊泡比色法检测I型胶原蛋白的高效检测手段。方法:以10,12-二十五碳二炔酸(PCDA)、10,12-二十五碳二炔-1-醇(PCDO)为原料,根据不同的摩尔比(10∶0、7∶3、6∶4)制备了功能化聚联乙炔囊泡(PDA-PDO囊泡),探讨了功能化PDA-PDO囊泡检测I型胶原蛋白的灵敏度、形貌与变色间关系、特异性、稳定性。结果:以PCDA和PCDO摩尔比为7∶3制备的聚联乙炔囊泡检测效果最佳,检测限为3.88 ng/mL;PDA-PDO囊泡变色越明显,表面塌陷越严重,同时该生物传感器具有良好的特异性、稳定性。结论:该生物传感器为I型胶原蛋白的可视化检测提供了途径。Objective:To construct a functional polyacetylene vesicle colorimetric method for the detection of type I collagen.Methods:Functionalized polyacetylene vesicles(PDA-PDO vesicles)are prepared using 10,12-25-carbodiyne-1-ol(PCDO)and 10,12-25-carbodiyne-1-ol(PCDA)as raw materials according to different molar ratios(10∶0,7∶3,6∶4).The sensitivity,morphology-color change relationship,specificity,and stability of the functionalized PDA-PDO vesicles for detecting type I collagen are investigated.Results:The PDA-PDO vesicles prepared with a PCDA to PCDO molar ratio of 7 to 3 shows the best detection performance,with a detection limit of 3.88 ng/mL.The more obvious the color change of PDA-PDO vesicles,the more severe the surface collapse.At the same time,this biosensor has good specificity and stability.Conclusion:This biosensor provides a pathway for visual detection of type I collagen.

关 键 词：胶原蛋白 生物传感器 聚联乙炔 自组装 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]