Hypoglycemic activities of flowers of Xanthoceras sorbifolia and identification of anti-oxidant components by off-line UPLC-QTOF-MS/MS-free radical scavenging detection  

作　　者：Xiajing Xu Yongli Guo Menglin Chen Ning Li Yi Sun Shumeng Ren Jiao Xiao Dongmei Wang Xiaoqiu Liu Yingni Pan 

机构地区：[1]School of Chinese Materia Medica,Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City,Shenyang Pharmaceutical University,Shenyang 110016,China [2]Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China [3]School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China

出　　处：《Chinese Herbal Medicines》2024年第1期151-161,共11页中草药（英文版）

基　　金：the Fourth National Investigation of Chinese materia medica resources in Liaoning Province (LN2018017, LN2019019);Career Development Support Plan for Young and Middle-aged Teachers in Shenyang Pharmaceutical University (No. ZQN2021014);Key Laboratory of Marine Biogenetic Resources, Ministry of Natural Resources (No. HY202105)

摘　　要：Objective: To identify phytochemical constituents present in the extract of flowers of Xanthoceras sorbifolia and evaluate their anti-oxidant and anti-hyperglycemic capacities.Methods: The AlCl3colorimetric method and Prussian Blue assay were used to determine the contents of total flavonoids and total phenolic acids in extraction layers, and the bioactive layers was screened through anti-oxidative activity in vitro. The Waters ACQUITY UPLC system and a Waters ACQUITY UPLC BEH C18column(2.0 mm × 150 mm, 5 μm) were used to identify the ingredients. And anti-oxidative ingredients were screened by off-line UPLC-QTOF-MS/MS-free radical scavenging. The ameliorative role of it was further evaluated in a high-fat, streptozotocin-induced type 2 diabetic rat model and the study was carried out on NADPH oxidase(PDB ID: 2CDU) by molecular docking.Results: Combined with the results of activity screening in vitro, the anti-oxidative part was identified as the ethyl acetate layer. A total of 24 chemical constituents were identified by liquid chromatographymass spectrometry in the ethyl acetate layer and 13 main anti-oxidative active constituents were preliminarily screened out through off-line UPLC-QTOF-MS/MS-free radical scavenging. In vivo experiments showed that flowers of X. sorbifolia could significantly reduce the blood glucose level of diabetic mice and alleviate liver cell damage. Based on the results of docking analysis related to the identified phytocompounds and oxidase which involved in type 2 diabetes, quercetin 3-O-rutinoside, kaempferol-3-O-rhamnoside, isorhamnetin-3-O-glucoside, and isoquercitrin showed a better inhibitory profile.Conclusion: The ethyl acetate layer was rich in flavonoids and phenolic acids and had significant anti-oxidant activity, which could prevent hyperglycemia. This observed activity profile suggested X. sorbifolia flowers as a promising new source of tea to develop alternative natural anti-diabetic products with a high safety margin.

关 键 词：anti-hyperglycemic activity ANTI-OXIDANT flowers of Xanthoceras sorbifolia Bunge NADPH oxidase off-line UPLC-QTOF-MS/MS-free radical scavenging 

分 类 号：R284.1[医药卫生—中药学]