Protective Effects of Danmu Extract Syrup on Acute Lung Injury Induced by Lipopolysaccharide in Mice through Endothelial Barrier Repair  

作　　者：XU Han XU Si-cong LI Li-yan WU Yu-huang TAN Yin-feng CHEN Long LIU Pei LIANG Chang-fu HE Xiao-ning LI Yong-hui 

机构地区：[1]Hainan Provincial Key Lab of Research&Development on Tropic Herbs,Hainan Medical University,Haikou,571199,China [2]Department of Stomatology,the Second Affiliated Hospital of Hainan Medical University,Haikou,571199,China

出　　处：《Chinese Journal of Integrative Medicine》2024年第3期243-250,共8页中国结合医学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No.82160826);Hainan Provincial Natural Science Foundation of China (No.2019RC207)。

摘　　要：Objective:To investigate the effects of Danmu Extract Syrup(DMS) on lipopolysaccharide(LPS)-induced acute lung injury(ALI) in mice and explore the mechanism.Methods:Seventy-two male Balb/C mice were randomly divided into 6 groups according to a random number table(n=12),including control(normal saline),LPS(5 mg/kg),LPS+DMS 2.5 mL/kg,LPS+DMS 5 mL/kg,LPS+DMS 10 mL/kg,and LPS+Dexamethasone(DXM,5 mg/kg) groups.After pretreatment with DMS and DXM,the ALI mice model was induced by LPS,and the bronchoalveolar lavage fluid(BALF) were collected to determine protein concentration,cell counts and inflammatory cytokines.The lung tissues of mice were stained with hematoxylin-eosin,and the wet/dry weight ratio(W/D) of lung tissue was calculated.The levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and IL-1βin BALF of mice were detected by enzyme linked immunosorbent assay.The expression levels of Claudin-5,vascular endothelial(VE)-cadherin,vascular endothelial growth factor(VEGF),phospho-protein kinase B(p-Akt) and Akt were detected by Western blot analysis.Results:DMS pre-treatment significantly ameliorated lung histopathological changes.Compared with the LPS group,the W/D ratio and protein contents in BALF were obviously reduced after DMS pretreatment(P<0.05 or P<0.01).The number of cells in BALF and myeloperoxidase(MPO) activity decreased significantly after DMS pretreatment(P<0.05 or P<0.01).DMS pre-treatment decreased the levels of TNF-α,IL-6 and IL-1β(P<0.01).Meanwhile,DMS activated the phosphoinositide 3-kinase/protein kinase B(PI3K/Akt) pathway and reversed the expressions of Claudin-5,VE-cadherin and VEGF(P<0.01).Conclusions:DMS attenuated LPS-induced ALI in mice through repairing endothelial barrier.It might be a potential therapeutic drug for LPS-induced lung injury.

关 键 词：Danmu Extract Syrup acute lung injury inflammatory factors vascular endothelial growth factor phosphoinositide 3-kinase/protein kinase B Chinese medicine Nauclea officinalis 

分 类 号：R285.5[医药卫生—中药学]