云南省鼠疫静息期家鼠鼠疫疫源地鼠中致病性耶尔森菌的调查研究  

Investigation of pathogenic Yersinia pestis in rodents of Mus musculus plague foci during the quiescent period in Yunnan Province

作　　者：张海鹏[1] 吴鹤松[1] 苏超[1] 段彪[1] 王鹏[1] 钟佑宏[1] ZHANG Haipeng;WU Hesong;SU Chao;DUAN Biao;WANG Peng;ZHONG Youhong(Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention,Yunnan Institute for Endemic Disease Control and Prevention,Dali,Yunnan 671000,China)

机构地区：[1]云南省地方病防治所、云南省自然疫源性疾病防控技术重点实验室,云南大理671000

出　　处：《中国地方病防治》2024年第1期9-11,共3页Chinese Journal of Control of Endemic Diseases

基　　金：国家自然科学基金(NO.81560545);云南省科技创新人才培养对象(202305AD160011);云南省医学学科后备人才培养对象(H-2019003)。

摘　　要：目的了解处于静息状态的云南省家鼠鼠疫疫源地鼠中3种致病性耶尔森菌的分布情况并探讨其相互作用关系,探究鼠疫耶尔森菌微生态环境,为防治致病性耶尔森菌提供依据。方法2016-2017年,通过多重分层抽样法在云南省抽选10个家鼠鼠疫疫源县采集鼠盲肠标本,经4℃冷增菌后,采用PCR方法检测foxA基因、inv基因及caf1基因进行初筛并用耶尔森选择培养基分离致病性耶尔森菌;利用SPSS17.0软件进行统计分析,比较各地区携菌率差异。结果云南省10个疫源县共采集鼠类盲肠标本2865份,分离到74株小肠结肠炎耶尔森菌,分离率为2.58%(74/2865);未分离到鼠疫耶尔森菌和假结核耶尔森菌;结论云南省家鼠鼠疫疫源地中存在小肠结肠炎耶尔森菌的分布,经统计分析,各地的分离率不同,且越早进入静息期的鼠疫疫源地鼠中小肠结肠炎耶尔森菌分离率越低;未发现鼠疫耶尔森菌和假结核耶尔森菌。Objective To understand the distribution and interaction of three species of pathogenic Yersinia pestis in rodents of Mus musculus plague foci during the quiescent period in Yunnan,and to explore the microecological environment of Yersinia pestis,so as to provide basis for the prevention and control of pathogenic Yersinia pestis.Methods From 2016 to 2017,using a multi-layered random sampling method,cecal specimens of mice were collected from 10 Mus musculus plague foci counties in Yunnan.After enrichment at 4,the specimens were screened using PCR for foxA,inv,and cafl genes,and pathogenic Yersinia was isolated using Yersinia selective agar.Statistical analysis was conducted using SPSS 17.O software to compare the carrier rates across different regions.Results A total of 2865 rodent cecal samples were collected from 10 plague foci counties in Yunnan,74 strains of Yersinia enterocolitica were isolated,with an isolation rate of 2.58%(74/2865).No Yersinia pestis or Yersinia pseudotuberculosis were isolated.Conclusion Yersinia enterocolitica is present in rodents from plague foci in Yunnan.The isolation rates vary across different regions,and regions that entered the quiescent period earlier had lower isolation rates of Yersinia enterocolitica.No Yersinia pestis or Yersinia pseudotuberculosis were found.

关 键 词：致病性耶尔森菌 家鼠鼠疫疫源地 鼠 云南省 

分 类 号：R516.8[医药卫生—内科学]