液相色谱-串联质谱法检测鱼肉中氯霉素残留  被引量:3

Detection of Chloramphenicol Residues in Fish by Liquid Chromatography-Tandem Mass Spectrometry

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作  者:任宏彬 张鑫鑫 杨晓伟 贾晓婷 李霖 REN Hong-Bin;ZHANG Xin-Xin;YANG Xiao-Wei;JIA Xiao-Ting;LI Lin(Taiyuan Customs Technology Center,Taiyuan 030024)

机构地区:[1]太原海关技术中心,太原030024

出  处:《中国口岸科学技术》2024年第1期80-85,共6页China Port Science and Technology

基  金:海关总署科研项目(2020HK217)。

摘  要:本文建立了液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)检测鱼肉中氯霉素残留的分析方法。试样经乙酸乙酯提取,氮吹浓缩后通过QuEChERS净化,C_(18)色谱柱分离,以水和甲醇为流动相进行洗脱,电喷雾负离子模式检测,溶剂内标法定量。结果表明,氯霉素在1~20 ng/mL线性范围内线性关系良好,检出限为0.2μg/kg,定量限为0.5μg/kg,3个水平浓度加标下的平均回收率在88.0%~91.0%之间,相对标准偏差为3.39%~5.28%(n=6)。该方法具有前处理简便、基质效应低、准确性高等优点,可用于批量水产品中氯霉素药物残留检测。A method for the determination of chloramphenicol residues in fish by liquid chromatography-tandem mass spectrometry(LC-MS/MS) was established.Samples were extracted with ethyl acetate,concentrated with nitrogen blowing,purified by QuEChERS,separated with C_(18) chromatographic column,eluted with water and methanol as mobile phases,detected by negative mode of electrospray,and quantified by solvent internal standard method.The results showed that chloramphenicol had a good linear relationship within the range of 1-20 ng/mL,and the detection limit was 0.2 μg/kg,with the quantification limit 0.5 μg/kg.The average recovery rates at three levels of concentration ranged from 88.0%-91.0%,and the relative standard deviations(RSDs)were 3.39%-5.28%(n = 6).This method has the advantages of simple pretreatment,low matrix effect and high accuracy,and can be used for the detection of chloramphenicol drug residues in batch aquatic products.

关 键 词:LC-MS/MS 氯霉素 基质效应 QuEChERS净化 

分 类 号:O657.63[理学—分析化学] TS254.7[理学—化学]

 

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