芩蓟凉血合剂对痔病相近动物模型的影响  

Effect of Qinji Liangxue mixture on animal models of hemorrhoids

作　　者：周蒙恩 李鹏[1] 金文琪 王若琳 郭修田[1] ZHOU Mengen;LI Peng;JIN Wenqi;WANG Ruolin;GUO Xiutian(Department of Anorectal Surgery,Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China)

机构地区：[1]上海中医药大学附属市中医医院肛肠科,上海200071

出　　处：《中国实验动物学报》2024年第1期33-39,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：上海市医院中药制剂产业转化协同创新中心;上海中医药大学产业发展中心医养结合科创项目(YYKC-2021-01-140);上海市中医医院未来计划(WL-YBKM-2022003K,WLJH2021ZY-GZS003,WLJH2021ZY-MZY024);上海市卫生健康委员会中医非药物疗法示范中心建设项目(ZY(2021-2023)-0204-01);上海市卫生健康委员会中医药科研项目计划(2022QN011);上海市市级医院新兴前沿技术联合攻关项目(SHDC12017119)。

摘　　要：目的探讨芩蓟凉血合剂(Qinji Liangxue mixture,QLM)对抗痔作用的机制。方法分别使用巴豆油制剂(croton oil preparation,COP)、冰醋酸制备大鼠急性痔病动物模型,将80只SD雌性大鼠,随机分为COP组与冰醋酸组两组,每组40只。COP组,随机分为对照组1(CA组),模型组1(CB组),QLM低剂量组1-1(CC组),QLM高剂量组1-2(CD组),地奥司明组1(CE组),每组8只;冰醋酸组,随机分为对照组2(GA组),模型组2(GB组),QLM低剂量组2-1(GC组),QLM高剂量组2-2(GD组),地奥司明组2(GE组),每组8只。除对照组,其余组均使用COP或冰醋酸处理,随后使用不同药物治疗7 d。使用苏木精-伊红(HE)染色观察COP及冰醋酸诱导的肛门直肠组织形态学,使用ELISA检测COP诱导的大鼠血清IL-6、TNF-α水平,记录冰醋酸诱导的大鼠肛周溃疡面积。将24只C57BL/6小鼠随机分为对照组(A组),QLM低剂量组(B组),QLM高剂量组(C组),肾上腺色腙片组(D组),每组6只,连续治疗7 d,使用毛细管法及断尾法评价QLM的止血疗效。结果QLM能够显著改善痔病相近动物模型的病理损伤,减轻COP诱导的病变程度评分(P<0.05),降低血清IL-6、TNF-α水平(P<0.05),减少冰醋酸诱导的肛周溃疡面积(P<0.05),缩短凝血及出血时间(P<0.05)。结论QLM具有良好的抗痔活性,其作用可能是通过抗炎、止血、减少组织损伤实现的。Objective To explore the mechanism underlying the anti-hemorrhoid effects of Qingji Liangxue mixture(QLM).Methods Models of acute hemorrhoid in rats were established using a croton oil preparation(COP)and glacial acetic acid.A total of 80 SD female rats were randomly divided into two major groups:the COP group and the glacial acetic acid group,each consisting of 40 rats.In the COP group,rats were further randomly assigned to the following subgroups:control group 1(group CA),model group 1(group CB),low-dose QLM group 1-1(group CC),high-dose QLM group 1-2(group CD),and diosmin group 1(group CE),with eight rats in each subgroup.In the glacial acetic acid group,rats were randomly divided into the following subgroups:control group 2(group GA),model group 2(group GB),low-dose QLM group 2-1(group GC),high-dose QLM group 2-2(group GD),and diosmin group 2(group GE),with eight rats in each subgroup.Except for the control groups,all other subgroups were treated with COP or glacial acetic acid then treated with various drugs for 7 days.HE staining was used to observe anorectal histomorphology induced by COP and glacial acetic acid.ELISAs were used to measure serum levels of IL-6 and TNF-αinduced by COP.Additionally,the area of perianal ulcers induced by glacial acetic acid was recorded.Twenty-four C57 mice were randomly divided into a control group(group A),low-dose QLM group(group B),high-dose QLM group(group C),and adrenal chromazone tablet group(group D).Results QLM significantly improved the pathological injury of animal models with similar hemorrhoids,reduced the score of pathological changes induced by COP(P<0.05),reduced the serum levels of IL-6 and TNF-α(P<0.05),reduced the area of perianal ulcers induced by glacial acetic acid(P<0.05),and shortened the time of coagulation and bleeding(P<0.05).Conclusions QLM has a good anti-hemorrhoid activity,which may be achieved by anti-inflammatory effects,hemostasis,and reducing tissue damage.

关 键 词：芩蓟凉血合剂 痔病 中医药 冰醋酸 巴豆油 

分 类 号：Q95-33[生物学—动物学]