研究ELISA联合荧光定量PCR检测丙型肝炎病毒的效果  

Study of ELISA Combined with Fluorescent Quantitative PCR for Detection of Hepatitis C Virus

作　　者：刘波 陈昌兰 LIU Bo;CHEN Changlan(Department of Health Supervision,Tengzhou Center for Disease Control and Prevention,Tengzhou,Shandong Province 277599 China;Department of Laboratory,Tengzhou Center for Disease Control and Prevention,Tengzhou,Shandong Province,277599 China)

机构地区：[1]滕州市疾病预防控制中心卫生监督科,山东滕州277599 [2]滕州市疾病预防控制中心检验科,山东滕州277599

出　　处：《系统医学》2024年第2期79-82,共4页Systems Medicine

摘　　要：目的评估丙型肝炎病毒检测应用酶联免疫吸附试验(Enzyme Linked Immunosorbent Assay,ELISA)联合荧光定量聚合酶链式反应(Polymerase Chain Reaction,PCR)检测的应用价值。方法选取2022年8月—2023年8月滕州市疾病预防控制中心接诊的90例疑似丙型肝炎病毒患者为研究对象,采用ELISA联合荧光定量PCR检测,以丙型肝炎病毒(Hepatitis Virus C,HCV)RNA检测结果作为“金标准”,分析检测方式的应用价值。结果HCV RNA检测结果显示,阳性率为47.78%,ELISA检测的阳性率40.00%,荧光定量PCR检测发现阳性量率42.22%,联合检测的阳性率46.67%。ELISA检测与荧光定量PCR检测在灵敏度、特异度、准确度、阳性预测值以及阴性预测值方面比较,差异无统计学意义(P均>0.05)。联合检测的灵敏度为93.02%、准确度为94.44%、阳性预测值为95.24%以及阴性预测值为93.75%,显著高于ELISA检测的67.44%、76.67%、80.56%、74.07%,联合检测的灵敏度、准确度显著高于荧光定量PCR检测(76.74%、83.33%),差异有统计学意义(P均<0.05)。结论丙型肝炎病毒检测应用ELISA联合荧光定量PCR检测检出率高。Objective To evaluate the application value of enzyme-linked immunosorbent assay(ELISA)combined with fluorescence quantitative polymerase chain reaction(PCR)for hepatitis C virus detection.Methods 90 suspected hepatitis C virus patients treated by Tengzhou Center for Disease Control and Prevention from August 2022 to August 2023 were selected as the research objects.ELISA combined with fluorescence quantitative PCR test was used,and hepatitis virus C(HCV)RNA test results were taken as the"gold standard"to analyze the application value of the de⁃tection method.Results The HCV RNA test results showed that the positive rate was 47.78%,the positive rate of the ELISA test was 40.00%,the Quantitative fluorescence PCR found a 42.22%positive rate,the positive rate of the com⁃bined test was 46.67%.The differences between ELISA and quantitative PCR in sensitivity,specificity,accuracy,posi⁃tive and negative predictive value were not statistically significant(all P>0.05).The sensitivity of the combined test was 93.02%,with an accuracy of 94.44%,a positive predictive value of 95.24%and a negative predictor of 93.75%,which were significantly higher than the 67.44%,76.67%,80.56%,74.07%detected by ELISA;the sensitivity and accuracy of the combined test were significantly higher than that of the fluorescence quantitative PCR test(76.74%,83.33%),and the differences were statistically significant(all P<0.05).Conclusion The application of ELISA com⁃bined with fluorescence quantitative PCR for the detection of hepatitis C virus has a high detection rate.

关 键 词：酶联免疫吸附试验 联合检测 荧光定量聚合酶链式反应 丙型肝炎病毒 

分 类 号：R4[医药卫生—临床医学]