过氧化氢诱导膜荚黄芪不定根毛蕊异黄酮葡萄糖苷积累的转录组分析  

Transcriptome Analysis Reveals Mechanisms of Calycosin-7-O-β-D-Glucoside Accumulation in Astragalus membranaceus Adventitious Roots through Hydrogen Peroxide

作　　者：窦维泽 姜雯 冯艺川 金卓 全雪丽[2] 吴松权[1,2] Dou Weize;Jiang Wen;Feng Yichuan;Jin Zhuo;Quan Xueli;Wu Songquan(College of Integration Science,Yanbian University,Yanji 133000,Jilin,China;Agricultural College,Yanbian University,Yanji 133000,Jilin,China)

机构地区：[1]延边大学融合学院,吉林延吉133000 [2]延边大学农学院,吉林延吉133000

出　　处：《作物杂志》2024年第1期48-56,共9页Crops

基　　金：吉林省自然科学基金(YDZJ202101ZYTS197);国家自然科学基金(21462044)。

摘　　要：H2O2处理能显著增加膜荚黄芪不定根中毛蕊异黄酮葡萄糖苷(CG)的含量。为探究其生物合成机制,以膜荚黄芪不定根为试验材料,经H_(2)O_(2)处理后进行转录组测序筛选差异表达基因。选取上调基因进行功能和代谢通路分析,用qRT-PCR验证,并对相关生理生化指标进行测定。结果表明,差异表达基因的筛选和基因功能注释表明,H_(2)O_(2)处理引起了氧化胁迫,为抵消氧化胁迫,膜荚黄芪不定根通过激活乙烯信号通路,诱导苯丙烷异黄酮生物合成途径中的全部结构基因,从而显著增加CG的含量。此外,为了增加CG,H_(2)O_(2)处理也会诱导苯丙氨酸的生物合成。试验结果进一步揭示了膜荚黄芪不定根中CG的积累机制,为今后规模化生产黄芪CG奠定理论依据。Hydrogen peroxide(H 2 O 2)significantly enhanced the content of calycosin-7-O-β-D-glucoside(CG)in Astragalus membranaceus adventitious roots(AMARs).To discover its biosynthetic mechanism of CG,AMARs were used to screen the differentially expressed genes(DEGs)through transcriptome sequencing after H_(2)O_(2) treatment.The function and metabolic pathways were analyzed by using up-regulated genes,verified them by qRT-PCR method,and measured the relevant physiological and biochemical parameters.The results showed that AMARs activated ethylene signaling,induced all structural genes in the phenylpropane-isoflavone biosynthesis pathway,significantly increased the content of CG to counteract the oxidative stress caused by H_(2)O_(2 )treatment.Furthermore,H_(2)O_(2) treatment also induced phenylalanine biosynthesis in order to increase the content of CG.These results revealed the accumulation mechanism of CG in AMARs which laid a theoretical basis for large-scale production of CG in the future.

关 键 词：H_(2)O_(2) 氧化胁迫 膜荚黄芪 转录组测序 毛蕊异黄酮葡萄糖苷 

分 类 号：S567.239[农业科学—中草药栽培]