45份糯高粱种质资源主要农艺性状和SSR标记的遗传多样性分析  

Genetic Diversity Analysis of 45 Glutinous Sorghum Germplasms Based on Major Agronomic Traits and SSR Markers

作　　者：孙远涛 龙文靖 李元[1,2] 刘天朋 赵甘霖[1,2] 丁国祥 倪先林[1,2] Sun Yuantao;Long Wenjing;Li Yuan;Liu Tianpeng;Zhao Ganlin;Ding Guoxiang;Ni Xianlin(Rice and Sorghum Research Institute,Sichuan Academy of Agricultural Sciences,Deyang 618000,Sichuan,China;Sichuan Branch of Chinese National Center for Sorghum Improvement,Luzhou 646000,Sichuan,China)

机构地区：[1]四川省农业科学院水稻高粱研究所,四川德阳618000 [2]国家高粱改良中心四川分中心,四川泸州646000

出　　处：《作物杂志》2024年第1期57-64,共8页Crops

基　　金：四川省科技计划(2022ZDZX0016);国家现代农业产业技术体系建设专项(CARS-06);四川省“十四五”农作物及蓄禽育种攻关(2021YFYZ0017);四川省财政自主创新专项“西南地区高粱种质资源鉴定评价与应用”(2022ZZCX068)。

摘　　要：糯高粱是酿造优质白酒的重要原料,为挖掘优异糯高粱资源材料,对45份不同来源的糯高粱种质资源10个主要农艺性状进行了分析,并用40对SSR引物解析糯高粱的遗传变异。结果表明,10个农艺性状的变化幅度、变异系数和遗传多样性指数均较大,42.2%的农艺性状间呈极显著相关性;40对SSR引物共检测到98个等位基因,多态信息含量变幅为0.044~0.690,其Shannon指数分布范围为0.1105~1.3626,遗传相似系数范围为0.235~0.980。结果表明本研究的45份糯高粱资源具有较为丰富的遗传多样性。基于主要农艺性状和SSR分子标记的UPGMA聚类分析分别将45份糯高粱资源划分为6大类和4大类。2种方法的聚类结果一致性较差,但可互为补充。研究结果为糯高粱种质资源的创新和新品种培育提供了理论参考,同时筛选出了一批优异的糯高粱资源材料。Glutinous sorghum is an important raw material for brewing high-quality liquor.In order to excavate high quality glutinous sorghum resource materials,ten main agronomic traits of 45 glutinous sorghum germplasm resources from different sources were analyzed,and 40 pairs of SSR primers were used to analyze the genetic variation of glutinous sorghum.The results showed that the change range,coefficient of variation and diversity index of ten phenotypic traits were large,and 42.2%of agronomic traits were significantly correlated;A total of 98 alleles were detected in 40 pairs of primers,the change range of polymorphic information was 0.044-0.690,the Shannon-weaver distribution range was 0.1105-1.3626,and the genetic similarity coefficient range was 0.235-0.980,which showed that 45 glutinous sorghum resources had rich genetic diversity.Based on the classification of main agronomic traits and molecular markers,45 waxy sorghum samples were divided into six categories and four categories by UPGMA cluster,respectively.The clustering results of the two methods were in poor agreement,but could complement each other.The results provide a theoretical reference for the innovation of sorghum germplasm resources and the breeding of new varieties,and a number of excellent glutinous sorghum resources were screened.

关 键 词：糯高粱 种质资源 主要农艺性状 SSR分子标记 遗传多样性分析 

分 类 号：S514[农业科学—作物学]