基于纳米银的比色阵列传感器的构建及在蛋白质检测中的应用  

Construction of Colorimetric Sensor Array Based on Silver Nanoparticles and Its Application in Protein Detection

作　　者：潘泓硕 徐嘉谊 陈信允 姜靓 徐香玉 Pan Hongshuo;Xu Jiayi;Chen Xinyun;Jiang Liang;Xu Xiangyu(College of clinical medicine;College of Basic Medicine,Jining Medical University,Jining,272067)

机构地区：[1]济宁医学院临床医学院,济宁272067 [2]济宁医学院基础医学院,济宁272067

出　　处：《化学通报》2024年第3期361-366,共6页Chemistry

基　　金：济宁医学院国家自然科学基金培育基金项目(JYP2018KJ03,JYP2018KJ17);济宁医学院校级本科教学改革研究项目(yb202219);济宁医学院大学生创新训练计划项目(cx2023053z,cx2023060z)资助。

摘　　要：蛋白质的快速高效检测和鉴定在医学诊断、不同疾病的治疗和蛋白质组学中具有巨大的前景。目前的检测手段大多存在一些问题,如操作繁琐、效率低等,因此开发一个理想的蛋白质检测方法尤为重要。以纳米银(AgNPs)为传感元件的阵列传感器在蛋白质检测方面具有操作便捷、准确率高、可视化等优点。本文合成两种不同颜色和形状的AgNPs:黄色球形和蓝色三角形,以此构建一个简单的比色阵列传感器,用于蛋白质的区分检测。该传感器可以准确地识别和区分不同种类的蛋白质,准确率为100%。在成功识别出不同类型的蛋白质的基础上,进一步评估了该阵列传感器应用于区分正常和变性蛋白质的能力,准确率为96.0%。此外,该阵列传感器对于未知样本的识别也具有高的准确率。Efficient and rapid detection and characterization of proteins can aid in medical diagnosis,treatment of different diseases,and proteomics.However,the current assays often have issues such as low efficiency and complicated operation.Therefore,the need for an ideal protein detection method is vital.Sensor array that use silver nanoparticles(AgNPs)as the sensing element have become popular due to their easy operation,high accuracy,and visualization in protein detection.In this paper,two AgNPs with different colors and shapes:yellow spheres and blue triangles,were synthesized to construct a simple colorimetric sensor array for protein discrimination detection.This sensor was successfully used to accurately identify and distinguish various types of proteins with an accuracy rate of 100%.Additionally,the sensor array was evaluated for its ability to differentiate between normal and denatured proteins,achieving an accuracy rate of 96.0%.Furthermore,the sensor array can also accurately recognize and detect unknown samples.

关 键 词：纳米银 比色阵列传感器 蛋白质检测 

分 类 号：R313[医药卫生—基础医学] O657.3[理学—分析化学]