姜黄素纳米颗粒联合可注射水凝胶用于改善心肌梗死后微环境的体外效果评价研究  

Evaluation Study of in Vitro Effect of Curcumin Nanopartrticle Combined with Injectable Hydrogels for Improving Microenvironment After Myocardial Infarction

作　　者：岳田 黄刚 杨佳丽 何建 旦增顿珠[3] 高寒 秦珊珊 侯君 徐俊波[1] YUE Tian;HUANG Gang;YANG Jiali;HE Jian;Danzeng Dunzhu;GAO Han;QIN Shanshan;HOU Jun;XU Junbo(Department of Cardiology,The Third People’s Hospital of Chengdu,Chengdu 610014,Sichuan,China;School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,Sichuan,China;Medical College,Tibet University,Lhasa 850000,Xizang,China)

机构地区：[1]成都市第三人民医院心内科,四川成都610014 [2]西南交通大学生命科学与工程学院,四川成都610031 [3]西藏大学医学院,西藏拉萨850000

出　　处：《心血管病学进展》2024年第2期187-192,共6页Advances in Cardiovascular Diseases

基　　金：国家自然科学基金(81900339);四川省博士后特别资助(TB2023095);西藏自治区自然科学基金重点项目(XZ202201ZR0036G);成都市第三人民医院1类孵化项目(CSY-YN-01-2023-003);成都市医学科研项目(2022038)。

摘　　要：目的为探索心肌梗死后的新治疗策略,本研究结合生物材料探索心肌梗死后恶劣微环境的改善策略。方法通过对生物相容的透明质酸改性与羧甲基壳聚糖共同制备可注射水凝胶,同时制备纳米胶束包载的姜黄素(PP@Cur)用以改善心肌细胞的凋亡。通过流式细胞仪检测、荧光染色验证PP@Cur的微环境改善作用以及心肌细胞保护作用。结果姜黄素纳米颗粒结合水凝胶能够改善心肌梗死后的高炎症和高活性氧环境。结论生物材料结合纳米递送平台用于心肌梗死后微环境改善是可行的,并且有望成为心肌梗死治疗的重要研究方向。Objective In order to explore new therapeutic strategies after myocardial infarction,this study explores the improvement of the harsh microenvironment after myocardial infarction by combining biomaterials.Methods Injectable hydrogels were prepared by biocompatible modified hyaluronic acid and carboxymethyl chitosan,and curcumin nanomicelles(PP@Cur)were prepared to correct the cardiomyocyte apoptosis.The microenvironmental ameliorating effect and cardiomyocyte protective effect of PP@Cur were verified by flow cytometry and fluorescence staining.Results Curcumin nanoparticles combined with hydrogel can correct the high inflammation and high reactive oxygen species environment after myocardial infarction.Conclusion The combination of biomaterials and nano delivery platforms for improving the microenvironment after myocardial infarction hearts is feasible,and has potential to become an important research direction for the treatment of myocardial infarction.

关 键 词：心肌梗死 微环境 炎症 活性氧 心肌细胞凋亡 

分 类 号：R542.22[医药卫生—心血管疾病]