紫菜薹线粒体基因组SSR分布特征的比较分析  

Characteristics of SSRs in Zicaitai Mitochondrial Genome

作　　者：肖婉钰 周贤玉 任海龙 黄红弟 郑岩松 张晶 许东林 XIAO Wanyu;ZHOU Xianyu;REN Hailong;HUANG Hongdi;ZHENG Yansong;ZHANG Jing;XU Donglin(Guangzhou Institute of Agriculture Science,Guangzhou,Guangdong 510308,China;Crop Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Crops Genetics and Improvement,Guangzhou,Guangdong 510640,China;Guangdong Provincial Improved Variety Introduce Service Corp.,Guangzhou,Guangdong 510091,China)

机构地区：[1]广州市农业科学研究院,广东广州510308 [2]广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室,广东广州510640 [3]广东省良种引进服务公司,广东广州510091

出　　处：《福建农业学报》2024年第1期49-56,共8页Fujian Journal of Agricultural Sciences

基　　金：2022年省级乡村振兴战略专项资金种业振兴项目(2022-NPY-03-001);广东省农业科技创新及推广项目(2023KJ133)。

摘　　要：【目的】分析紫菜薹(Brassica rapa var.purpuraria)线粒体基因组SSR序列的分布特征,并与芸薹属主要物种进行比较,为紫菜薹SSR分子标记的开发及遗传进化研究提供参考。【方法】利用MISA软件对紫菜薹及芸薹属6个基本种(包括白菜3个变种)的线粒体基因组进行搜索,并对搜索到的SSR序列分布特征进行比较分析。【结果】在紫菜薹及芸薹属6个基本种(包括白菜3个变种)的线粒体基因组中,分别筛选到168、170、167、168、180、280、185、165、179个完整的SSR序列,相对密度分别为764、774、760、764、775、777、721、744、771个·Mb-1,SSR序列的总长度分别为1562、1577、1547、1562、1664、2564、1722、1524、1646 bp,占各自线粒体基因组序列总长度的比例分别为0.71%、0.72%、0.70%、0.71%、0.72%、0.71%、0.67%、0.69%和0.71%。在1~6个不同核苷酸重复单元中,紫菜薹及芸薹属6个基本种的SSR序列均是单核苷酸重复单元最多,然后依次是二核苷酸、四核苷酸、三核苷酸、五核苷酸,均未发现六核苷酸重复单元。其中,A/T、AG/CT、AT/AT和C/G是芸薹属线粒体基因组共有的常见重复单元类型。【结论】紫菜薹线粒体基因组大小为219779 bp,共筛选出168个SSR分子标记,相对密度为764个·Mb-1,平均长度为9 bp,以单核苷酸重复单元的数量最多,其次为二、四核苷酸重复单元类型,具有较大的多态性标记开发潜力。【Objective】The simple sequence repeats(SSRs)in the mitochondrial genome of zicaitai(Brassica rapa var.Purpuraria)were compared with those of other species in the genus.【Method】MISA software was used to search the mitochondrial genomes of zicaitai as well as 5 other major species and 3 mutants of Brassica rapa.The SSR sequence distributions of the specimens were compared and analyzed.【Result】In the mitochondrial genomes,168,170,167,168,180,280,185,165,and 179 complete SSRs were screened.The sequences showed relative densities of 764,774,760,764,775,777,721,744,and 771 per Mb with total lengths of 1562,1577,1547,1562,1664,2564,1722,1524,and 1646 bp that accounted for 0.71%,0.72%,0.70%,0.71%,0.72%,0.71%,0.67%,0.69%,and 0.71%of the total sequence length of each specimen,respectively.Among the 1-6 different nucleotide repeat units in zicaitai and the other major Brassica species,most SSRs were mononucleotide,which was followed by dinucleotide,tetranucleotide,trinucleotide,and pentanucleotide,but no hexonucleotide.A/T,AG/CT,AT/AT,and C/G were the common repeat unit types in them.【Conclusion】The mitochondrial genome of zicaitai was 219779 bp with 168 SSRs at the relative density of 764 markers/Mb and 9 bp in length.Mono-,di-,and tetra-nucleotides were the most abundant repeat units,which could potentially be developed as highly polymorphic markers for species differentiation.

关 键 词：紫菜薹 芸薹属 线粒体 SSR 

分 类 号：S634.6[农业科学—蔬菜学]