两种丛枝菌根真菌定殖青山杨后对舞毒蛾的抗性研究  

Effects of arbuscular mycorrhizal fungal colonization on Populus pseudo-cathayana×P.deltoides resistance to Lymantria dispar larvae

作　　者：赵佳齐 方静 谭明涛 武帅 任英杰 孟昭军[1] 严善春[1] Zhao Jiaqi;Fang Jing;Tan Mingtao;Wu Shuai;Ren Yingjie;Meng Zhaojun;Yan Shanchun(School of Forestry,Key Laboratory of Sustainable Forest Ecosystem Management of Ministry of Education,Northeast Forestry University,Harbin 150040,Heilongjiang,China)

机构地区：[1]东北林业大学林学院,森林生态系统可持续经营教育部重点实验室,黑龙江哈尔滨150040

出　　处：《北京林业大学学报》2024年第3期53-59,共7页Journal of Beijing Forestry University

基　　金：中央高校基本科研业务费专项(2572019CP11)。

摘　　要：【目的】研究丛枝菌根真菌(AMF)对青山杨抗虫性的影响以及舞毒蛾对AMF定殖青山杨的适应能力,为AMF定殖青山杨后抗虫性的研究提供科学依据。【方法】分别以对照(CK,无AMF定殖)、根内根孢囊霉(GI)或摩西管柄囊霉(GM)定殖的青山杨叶片饲喂舞毒蛾幼虫,统计3、4、5龄幼虫的体重、体长、头壳宽和食物利用率,测定4、5龄幼虫解毒酶酸性磷酸酯酶(ACP)和碱性磷酸酯酶(AKP)的活性。【结果】GI组3、4龄幼虫的体重显著高于对照组(P<0.05),5龄与对照组差异不显著,GM组5龄幼虫的体长、体重、头壳宽、食物利用率均显著高于对照组。GI组3龄幼虫取食量显著高于对照组(P<0.05),4、5龄显著低于对照组,GM组5龄显著高于对照组,3、4龄与对照组差异不显著。GI组ACP显著低于对照组(P<0.05),AKP在4龄显著高于对照组,5龄AKP活性显著低于对照组。GM组两种解毒酶活性均显著高于对照组。【结论】GI定殖的青山杨能促进舞毒蛾低龄幼虫的生长,对舞毒蛾老熟幼虫的生长影响不显著。GM定殖青山杨叶片促进了舞毒蛾幼虫的生长发育,提高了食物的利用效率,激发了幼虫体内磷酸酯酶活性,表明舞毒蛾对GM定植的青山杨有更好的适应性。AMF对树木及植食性昆虫的影响具有种类特异性,GI定殖对青山杨的抗虫性的影响呈中性,GM定殖对青山杨的抗舞毒蛾产生负效应。[Objective]This paper explores the effects of arbuscular mycorrhizal fungi(AMF)on the resistance of Qingshan poplar(Populus pseudo-cathayana×P.deltoides)to the gypsy moth(Lymantria dispar)larvae and the adaptability of L.dispar larvae to the AMF colonization,so as to provide a scientific basis for the study of insect resistance of Qingshan poplar colonized by AMF.[Method]The spongy moth larvae were fed with fresh leaves of Qingshan poplar seedlings,which were treated with Glomus intraradices or G.mosseae or untreated(CK:control).The body mass,length,head capsule width and food utilization of the 3rd,4th and 5th instars of L.dispar larvae were measured,and the activities of acid phosphatase(ACP)and alkaline phosphatase(AKP)of the 4th and 5th instars of larvae were determined.[Result]The body mass of the 3rd/4th instar larvae in GI group was significantly higher than that in the control group,but there was no significant difference in the body mass of the 5th instar larvae between the GI group and the control group.The body length,mass,head capsule width and food utilization of the 5th instar larvae in the GM group were significantly higher than those in the control group.Food intake of the 3rd instar larvae in the GI group was significantly higher than that in the control group,but the reverse was true for the 4th and 5th instars;the food intake of the 5th instar larvae in the GM group was significantly higher than that in the control group,but there was no significant difference in food intake of the 3rd and 4th instars between the GM group and the control group.The ACP activity of the 4th and 5th instars in the GI group was significantly lower than that in the control group,and AKP activity of the 4th instar in the GI group was significantly higher than that in the control group,whereas the reverse was true for the 5th instar.On the other hand,the activities of these two detoxifying enzymes in the GM group were significantly higher than those in the control group.[Conclusion]The GI treatment enhances the growth

关 键 词：丛枝菌根真菌 青山杨 舞毒蛾 生长发育 解毒酶活性 

分 类 号：S763.42[农业科学—森林保护学]