沙葱乙醇提取物对奶牛血浆生化、免疫、抗氧化和激素指标的影响  

Effects of Ethanol Extract of Allium mongolicum Regel on Plasma Biochemical,Antioxidant,Immune and Hormone Indexes of Dairy Cows

作　　者：李华北 段嘉钰 蒋林树[2] 敖长金[1] 白晨[1] LI Huabei;DUAN Jiayu;JIANG Linshu;AO Changjin;BAI Chen(Key Laboratory of Animal Nutrition and Feed Science of Colleges and Universities of Inner Mongolia,College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China;Beijing Key Laboratory of Dairy Cattle Nutrition,College of Animal Science and Technology,Beijing University of Agriculture,Beijing 102208,China)

机构地区：[1]内蒙古农业大学动物科学学院,内蒙古自治区高校动物营养与饲料科学重点实验室,呼和浩特010018 [2]北京农学院动物科学技术学院,北京农学院奶牛营养学北京市重点实验室,北京102208

出　　处：《动物营养学报》2024年第3期1734-1744,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：内蒙古农业大学青年教师科研能力提升专项(BR230102);北京农学院奶牛营养学北京市重点实验室开放课题(2020019);内蒙古农业大学动物科学学院高水平成果培育专项项目(QT202220)。

摘　　要：本试验旨在研究沙葱乙醇提取物对奶牛血浆生化、免疫、抗氧化和激素指标的影响。选取12头体况和产奶量相近、2~3胎次的泌乳中期荷斯坦奶牛,随机分为2组,每组6个重复,每个重复1头。对照组奶牛饲喂基础饲粮,试验组奶牛饲喂基础饲粮+54 g/d沙葱乙醇提取物。预试期14 d,正试期42 d。结果表明:1)2组之间血浆葡萄糖(GLU)、尿素(UREA)、总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)、肌酐(CR)、胆固醇(CHOL)含量及谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)活性均无显著差异(P>0.05)。2)试验组的血浆谷胱甘肽(GSH)含量和谷胱甘肽过氧化物酶(GSH⁃Px)活性显著高于对照组(P<0.05),血浆超氧化物歧化酶(SOD)活性极显著高于对照组(P<0.01)。2组之间血浆总抗氧化能力(T⁃AOC)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量均无显著差异(P>0.05)。3)试验组的血浆白细胞介素-2(IL⁃2)和白细胞介素-10(IL⁃10)含量显著高于对照组(P<0.05),血浆白细胞介素-6(IL⁃6)含量极显著低于对照组(P<0.01)。2组之间血浆免疫球蛋白A(IgA)、免疫球蛋白M(IgM)、免疫球蛋白G(IgG)、干扰素-γ(IFN⁃γ)、肿瘤坏死因子-α(TNF⁃α)含量均无显著差异(P>0.05)。4)2组之间血浆生长激素(GH)、催乳素(PRL)、雌二醇(E2)、胰岛素(INS)、皮质醇(HYD)含量均无显著差异(P>0.05)。由此可见,饲粮中添加54 g/d沙葱乙醇提取物能增强泌乳中期奶牛的免疫、抗氧化能力,且对奶牛血浆生化指标无不良影响。This experiment was conducted to study the effects of ethanol extract of Allium mongolicum Regel on plasma biochemical,antioxidant,immune and hormone indexes of dairy cows.Twelve 2 to 3 parity midlactation Holstein cows with similar body condition and milk production were selected and randomly divided into 2 groups with 6 replicates in each group and 1 cow in each replicate.Cows in the control group were fed a basal diet,and others in the test group were fed the basal diet+54 g/d ethanol extract of Allium mongolicum Regel.The preexperimental period lasted for 14 days,and the experimental period lasted for 42 days.The results showed as follows:1)there were no significant differences in glucose(GLU),urea(UREA),total protein(TP),albumin(ALB),globulin(GLB)contents and alanine aminotransferase(ALT),aspartate aminotransferase(AST)alkaline phosphatase(ALP)activities in plasma between 2 groups(P>0.05).2)The glutathione(GSH)content and glutathione peroxidase(GSHPx)activity in plasma of the test group were significantly higher than those of the control group(P<0.05),and the plasma superoxide dismutase(SOD)activity was significantly higher than that of the control group(P<0.01).There were no significant differences in total antioxidant capacity(TAOC),catalase(CAT)activity and malondialdehyde(MDA)content in plasma between 2 groups(P>0.05).3)The contents of interleukin2(IL2)and interleukin10(IL10)in plasma of the test group were significantly higher than those of the control group(P<0.05),and the plasma interleukin6(IL6)content was significantly lower than that of the control group(P<0.01).There were no significant differences in contents of immunoglobulin A(IgA),immunoglobulin M(IgM),immunoglobulin G(IgG),interferonγ(IFNγ)and tumor necrosis factorα(TNFα)in plasma between 2 groups(P>0.05).4)There were no significant differences in contents of growth hormone(GH),prolactin(PRL),estradiol(E2),insulin(INS),cortisol(HYD)in plasma between 2 groups(P>0.05).In conclusion,dietary adding 54 g/d ethanol extract of Allium mongolicum

关 键 词：沙葱乙醇提取物 奶牛 生化 免疫 抗氧化 激素 

分 类 号：S823[农业科学—畜牧学]