板蓝根与醋五味子对鸭坦布苏病毒感染雏鸭的治疗效果研究  

Study on the therapeutic effects of Isatis tinctoria and vinegar-treated Schisandra Chinensis on ducklings infected with Duck tambusu virus

作　　者：王晗晗 嵇辛勤[1,2] 阮涌[1,3] 邓珊珊 杨纯培 吴宗豪 姚碧琼 WANG Hanhan;JI Xinqin;RUAN Yong;DENG Shanshan;YANG Chunpei;WU Zonghao;YAO Biqiong(College of Animal Science,Guizhou University,Guiyang 550025,China;Guizhou Provincial Laboratory of Animal Disease,Guiyang 550025,China;Guizhou Provincial Key Laboratory of Animal Genetics,Breeding and Reproduction,Guiyang 550025,China;Qingzhen City Bureau of Agriculture and Rural Affairs,Qingzhen 551499,China;Sansui County Animal Disease Prevention and Control Center,Sansui 556500,China)

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]贵州省动物疫病研究室,贵阳550025 [3]贵州省动物遗传育种与繁殖重点实验室,贵阳550025 [4]清镇市农业农村局,贵州清镇551499 [5]三穗县动物疫病预防控制中心,贵州三穗556500

出　　处：《黑龙江畜牧兽医》2024年第5期100-106,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：贵州省科技计划项目(黔科合支撑[2020]1Y040号;黔科合支撑[2022]一般137)。

摘　　要：为了研究板蓝根及醋五味子对鸭坦布苏病毒(DTMUV)感染雏鸭的治疗效果,试验将120只7日龄DTMUV抗体阴性雏鸭平均分为空白对照组、病毒对照组、板蓝根组及醋五味子组,每组30只;饲养至15日龄时,除空白对照组外,其余各组攻毒DTMUV,同时两个药物组每日按体重口服相应药物1 g/kg,观察并记录各组临床症状及存活率并绘制生存曲线;在攻毒后第3,6,9,12天每组处死3只雏鸭,观察剖检病变并检测心脏、肝脏、脾脏和脑组织的病毒载量,并对第12天的上述组织进行切片、H.E.染色观察。结果表明:病毒对照组出现神经症状等典型临床症状并发生死亡,而板蓝根组及醋五味子组的临床症状不明显且无死亡,存活率显著高于病毒对照组(P<0.05)。病毒对照组脾脏肿大、出现大理石样花纹,心包有纤维素性渗出,脑膜严重充血、出血,肾脏苍白、出血;板蓝根组脾脏、心脏、大脑、肾脏的病变不明显;醋五味子组脾脏出现大理石样花纹,脑膜轻微充血。病毒对照组肝细胞严重空泡变性、坏死,大脑见小胶质细胞结节,脾脏淋巴细胞明显减少,板蓝根组及醋五味子组肝细胞变性程度减轻,大脑未见小胶质细胞结节,脾脏淋巴细胞数增加,其中板蓝根组效果较好。第3天时,板蓝根组及醋五味子组脾脏和肝脏的病毒载量极显著或显著低于病毒对照组(P<0.01或P<0.05);第9,12天时,板蓝根组及醋五味子组心脏、肝脏、脾脏、大脑组织病毒载量显著或极显著低于病毒对照组(P<0.05或P<0.01),其中板蓝根组效果更好。说明板蓝根与醋五味子均可以缓解DTMUV感染引起的机体损伤,降低组织病毒载量及雏鸭死亡率,其中板蓝根效果更好。In order to study the therapeutic effects of Isatis tinctoria and vinegar-treated Schisandra Chinensis on ducklings infected with Duck tambusu virus(DTMUV),in the experiment,120 DTMUV antibody-negative ducklings at 7 days of age were divided into blank control group,virus control group,Isatis tinctoria group and vinegar-treated Schisandra Chinensis group,with 30 ducklings in each group.When the ducks were reared to 15 days of age,all groups except the blank control group were challenged with DTMUV,and the two drug groups were orally administered 1 g/kg of the respective drugs according to their body weight every day;the clinical symptoms and survival rates of each group were observed and recorded,and the survival curves were plotted.Three ducklings in each group were sacrificed on the 3rd,6th,9th and 12th days post virus challenge,and the lesions were observed by dissection,and the viral loads of heart,liver,spleen and brain tissues were detected,and the above tissues on the 12th day were sectioned and observed after H.E.staining.The results showed that typical clinical symptoms such as neurological symptoms and death occurred in virus control group,while the clinical symptoms were not obvious and there was no death in Isatis tinctoria group and vinegar-treated Schisandra Chinensis group,and the survival rates were significantly higher than that of virus control group(P<0.05).In the virus control group,the spleen was enlarged and appeared marbled,the pericardium had fibrinous exudation,the meninges were severely congested and hemorrhagic,and the kidneys were pale and hemorrhagic.The lesions of spleen,heart,brain,and kidney were not obvious in the Isatis tinctoria group;the spleen appeared marbled and the meninges were slightly congested in the vinegar-treated Schisandra Chinensis group.In the virus control group,hepatocytes were severely vacuolated and necrotic,microglia nodules were seen in the brain,and the number of splenic lymphocytes was significantly reduced.The degree of hepatocyte degeneration was reduced

关 键 词：鸭坦布苏病毒 中草药 雏鸭 抗病毒 治疗效果 

分 类 号：S855.3[农业科学—临床兽医学]