肝性脑病大鼠经粪便细菌移植后的肠道菌群宏基因组分析  

Macrogenomic analysis of intestinal flora in rats with hepatic encephalopathyafter fecal microbiota transplantation

作　　者：王威巍 刘丰睿 吕朝华 申呈祥 WANG Weiwei;LIU Fengrui;LYU Chaohua;SHEN Chengxiang(Department of Hepatobiliary Surgery,Tongliang District People′s Hospital of Chongqing,Chongqing 402560,China;Department of Hepatobiliary Surgery,the Second Affiliated Hospital of the Army Medical University,Chongqing 400037,China)

机构地区：[1]重庆市铜梁区人民医院肝胆胰外科,重庆402560 [2]陆军军医大学第二附属医院肝胆外科,重庆400037

出　　处：《现代医药卫生》2024年第6期913-917,共5页Journal of Modern Medicine & Health

基　　金：重庆市科卫联合医学项目(2022MSXM044)。

摘　　要：目的建立肝性脑病大鼠模型及粪便细菌移植(FMT)模型,探索细菌移植后肝性脑病大鼠肠道菌群的改变。方法选择30只SD大鼠,采用四氯化碳联合乙醇的方法建立肝性脑病大鼠模型。将大鼠随机分成3组,A组为正常对照组,B组为肝性脑病模型组,C组为FMT组,每组10只。B组进行同等剂量生理盐水灌胃,C组进行FMT(按200μL/d灌胃,每毫升细菌溶液含细菌1.5×10^(11))。每天观察大鼠生存情况。收集大鼠肠道粪便进行宏基因组测序。结果实验过程中大鼠共死亡9只,A组无死亡。FMT前后,C组大鼠移植后体重明显增加。通过宏基因组测序,发现C组较B组可有效地改善菌种的多样性和丰度;与A组比较,B、C组中变形菌门含量显著升高,拟杆菌门丰度显著降低。结论FMT可以改变肝性脑病大鼠肠道菌群的结构。Objective To establish a rat model of hepatic encephalopathy and fecal microbiota transplantation(FMT)model,and explore the effects of bacterial transplantation on intestinal flora in rats with hepatic cirrhosis.Methods A total of 30 SD rats were selected and rat liver models were established using carbon tetrachloride+ethyl alcohol method.The rats were randomly divided into three groups:group A as the normal control group,group B as the hepatic encephalopathy model group,and group C as the FMT group,with 10 rats in each group.Group B was given the same dose of normal saline,and group C was given FMT transplantation(200μL/d,bacteria 1.5×10^(11) per mL of bacterial solution).The survival of the rats was observed daily.The intestinal feces of rats were collected for metagenomic sequencing.Results A total of nine rats died during the experiment,while group A did not die.Before and after FMT,the body weight of rats in group C increased significantly after transplantation.Through metagenomic sequencing,it was found that group C could effectively improve the diversity and abundance of bacteria compared with group B.Compared with group A,the content of Proteobacteria in group B and C was significantly increased,while the abundance of Bacteroides was significantly decreased.Conclusion FMT can alter the structure of intestinal flora in rats with hepatic encephalopathy.

关 键 词：粪便细菌移植 肝性脑病 宏基因组 肠道菌群 大鼠 

分 类 号：R333.4[医药卫生—人体生理学] R378.2[医药卫生—基础医学] R575.3