高效液相色谱法测定肝复颗粒中6种成分含量  

Determination of Six Components in Ganfu Granules by High Performance Liquid Chromatography

作　　者：吴国军[1] 徐惠玲[1] 龚艳珍 Wu Guojun;Xu Huiling;Gong Yanzhen(Jiangxi Provincial People's Hospital,Nanchang Jiangxi 330006,China)

机构地区：[1]江西省人民医院,江西南昌330006

出　　处：《医疗装备》2024年第5期1-4,8,共5页Medical Equipment

基　　金：江西省中医药管理局科技计划(2022B1056)。

摘　　要：目的建立高效液相色谱法(HPLC)法,同时测定肝复颗粒中栀子苷、西红花苷Ⅰ、丹参酮Ⅰ、隐丹参酮、虎杖苷、丹参酮ⅡA 6种成分的含量。方法采用COSMOSILC_(18)色谱柱,以乙腈-0.1%磷酸为流动相,流速为0.8 ml/min,进行梯度洗脱,检测波长为205 nm。结果6种成分均实现较好的分离,在一定的浓度范围内各成分均呈良好的线性关系,平均回收率为97.92%~99.67%,相对标准偏差(RSD)为0.28%~1.51%。定量数据经统计学软件分析,可知栀子苷和西红花苷Ⅰ是影响批次样品差异的主要因素。结论建立HPLC法同时测定肝复颗粒中栀子苷、西红花苷Ⅰ、丹参酮Ⅰ、隐丹参酮、虎杖苷、丹参酮ⅡA 6种成分的含量,有利于对肝复颗粒成分的质量控制。Objective To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of six components in Ganfu granules,including geniposide,crocinⅠ,tanshinoneⅠ,cryptotanshinone,polygonatum cuspidatum glycoside,and tanshinoneⅡA.Methods Used a COSMOSILC_(18) chromatographic column,with acetonitrile 0.1%phosphoric acid as the mobile phase and a flow rate of 0.8 ml/min,for gradient elution with a detection wavelength of 205 nm.Results Six components all achieved good separation,and within a certain concentration range,each component showed a good linear relationship.The average recovery rate was from 97.92%to 99.67%,and the RSD was from 0.28%to 1.51%.Quantitative data was analyzed by statistical software,and it was found that geniposide and crocinⅠwere the main factors affecting the differences in batch samples.Conclusion Establishing a high-performance liquid chromatography(HPLC)method to simultaneously determine the content of six components in Ganfu granules,including geniposide,crocinⅠ,tanshinoneⅠ,cryptotanshinone,polygonatum cuspidatum glycoside,and tanshinoneⅡA,is beneficial for the quality control of Ganfu granules.

关 键 词：肝复颗粒 6种成分 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]