五味苦参肠溶胶囊对溃疡性结肠炎大鼠的疗效及作用机制研究  

Effect and mechanism of Five-Flavor Sophora Flavescens Enteric-Coated Capsules on rats with ulcerative colitis

作　　者：熊加彬 李珍[1] 吴俊俊 李春霞[1] 王宇芳[1] 李华铭[1] XIONG Jiabin;LI Zhen;WU Junjun;LI Chunxia;WANG Yufang;LI Huaming(Department of Gastroenterology,Hangzhou Third People's Hospital,Hangzhou 310009,China;不详)

机构地区：[1]杭州市第三人民医院消化内科,310009

出　　处：《浙江医学》2024年第5期464-469,I0004,共7页Zhejiang Medical Journal

基　　金：浙江省中医药科技计划项目(2022ZA138)。

摘　　要：目的 探讨五味苦参肠溶胶囊对溃疡性结肠炎(UC)大鼠的疗效及作用机制。方法 采用3%葡聚糖硫酸钠建立UC大鼠模型,造模成功后采用随机数字表法将大鼠随机分为空白组、模型组、美沙拉嗪组及五味苦参肠溶胶囊组,每组10只,每日给予0.9%氯化钠溶液或相应药物灌胃,连续2周,观察并比较各组大鼠疾病活动指数(DAI)评分、结肠黏膜损伤指数(CMDI)评分,采用ELISA法测定血清IL-6、IL-10水平,qRT-PCR法检测结肠组织IL-6、IL-10 mRNA表达情况,免疫组化法检测结肠组织内黏液蛋白2(MUC2)表达水平,采用髓过氧化物酶比色法检测结肠组织髓过氧化物酶(MPO)活性。结果 与空白组相比,模型组大鼠DAI评分、CMDI评分、血清IL-6水平及结肠组织IL-6 mRNA表达水平、MPO活性均升高(均P<0.05),血清IL-10水平及结肠组织IL-10 mRNA、MUC2表达水平均下降(均P<0.05)。与模型组相比,美沙拉嗪组和五味苦参肠溶胶囊组大鼠DAI评分、CMDI评分、血清IL-6水平及结肠组织IL-6 mRNA表达水平、MPO活性均下降(均P<0.05),血清IL-10水平及结肠组织IL-10 mRNA、MUC2表达水平均升高(均P<0.05)。结论 五味苦参肠溶胶囊可能通过降低促炎因子IL-6水平和升高抗炎因子IL-10水平、上调MUC2表达量、降低MPO活性来减轻UC大鼠炎症反应,促进肠黏膜修复。Objective To study the effect and mechanism of Five-Flavor Sophora Flavescens Enteric-Coated Capsules on rats with ulcerative colitis(UC).Methods The UC rat model was established by 3% dextran sulfate sodium(DSS).After successful modeling,the rats were randomly divided into blank group,model group,mesalazine group and FiveFlavor Sophora Flavescens Enteric-Coated Capsules group,with 10 in each group.The rats were given normal saline or corresponding drugs by intragastric administration every day for two consecutive weeks.Disease activity index(DAI) score and colonic mucosal damage index(CMDI) score were observed and compared among groups,and the expression levels of interleukin(IL-6) and interleukin(IL-10) in serum and expressions of IL-6 and IL-10 m RNA in colon tissues were detected by enzyme-linked immunosorbent assay(ELISA) and fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR),respectively.The expression level of mucin 2(MUC2) and the activity of myeloperoxidase(MPO) in colon tissues were determined by myeloperoxidase colorimetry method,respectively.Results Compared with blank group,DAI score,CMDI score,serum IL-6 content,IL-6 mRNA expression in colon tissue,and MPO activity in model group were all significantly increased(all P<0.05),while IL-10 content,IL-10 mRNA expression in colon tissue,and average optical density(AOD) of MUC2 decreased significantly(all P<0.05).Compared with model group,DAI score,CMDI score,serum IL-6content,IL-6 mRNA expression in colon tissue,and MPO activity of rats in mesalazine group and Five-Flavor Sophora Flavescens Enteric-Coated Capsules group were significantly decreased(all P<0.05),while the content of IL-10,IL-10mRNA expression in colon tissue,and AOD value of MUC2 were significantly increased(all P<0.05).Conclusion FiveFlavor Sophora Flavescens Enteric-Coated Capsules may promote intestinal mucosal repair and alleviate inflammation in UC rats by decreasing the level of pro-inflammatory factor IL-6 and increasing the level of anti-inflammatory

关 键 词：溃疡性结肠炎 五味苦参肠溶胶囊 炎症因子 

分 类 号：R285.5[医药卫生—中药学]