1-硝基芘通过自噬通路对秀丽隐杆线虫寿命的影响研究  被引量:1

Effects of 1-nitropyrene on lifespan of Caenorhabditis elegans through autophagy pathway

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作  者:付玉聪 王萌 刘盛杰 赵理乐[3] 张绪梅 张利文 FU Yucong;WANG Meng;LIU Shengjie;ZHAO Lile;ZHANG Xumei;ZHANG Liwen(Department of Labor Health and Environmental Hygiene,School of Public Health,Tianjin Medical University,Tianjin,300070,China;Department of Nutrition and Food Hygiene,School of Public Health,Tianjin Medical University,Tianjin,300070,China;Cerebral Department,Tianjin Xiqing Hospital,Tianjin,300380,China)

机构地区:[1]天津医科大学公共卫生学院劳动卫生与环境卫生学系,天津300070 [2]天津医科大学公共卫生学院营养与食品卫生学系,天津300070 [3]天津市西青医院脑系科,天津300380

出  处:《职业与健康》2024年第2期195-199,共5页Occupation and Health

基  金:国家环境保护机动车污染控制与模拟重点实验室开放基金(VECS2022K04);天津市西青医院天津医科大学科联体项目(XQYYKLT202102)。

摘  要:目的 本研究采用秀丽隐杆线虫(简称线虫)模型探讨1-硝基芘(1-nitropyrene,1-NP)对线虫寿命的影响和自噬在寿命影响中的作用。方法 在正常培养条件下,研究不同浓度的1-NP(0、10^(-4)、10^(-2)、10^(2)、10^(4)ng/L)亚急性干预5天对线虫寿命的影响,观察1-NP对线虫健康相关参数如体长、运动能力和脂褐素和寿命的影响。使用实时定量聚合酶链反应(quantitative real time-polymerase chain reaction,qRT-PCR)检测自噬相关标志基因(bec-1和lgg-1)的表达情况。结果 与对照组相比,1-NP可显著降低线虫的寿命,其中104ng/L剂量降低寿命效果最明显,降低率为28.2%。1-NP(10^(4)ng/L)可降低线虫的体长(P<0.05)和自噬关键基因表达水平(P<0.05),增加线虫体内脂褐素的蓄积(P<0.05)。结论 1-NP可以通过降低线虫体内自噬水平,进而使线虫的寿命缩短。Objective By using the Caenorhabditis elegans model(hereafter referred to as C.elegans),to investigate the effects of 1-nitropyrene(1-NP)on the lifespan of C.elegans and the role of autophagy in the impact of lifespan.Methods The effects of subacute exposure(five days)of 1-NP(0,10^(-4),10^(-2),10^(2),10^(4) ng/L)on the life span of C.elegans were studied under normal culture conditions.The effects of 1-NP on health indicators(body length,exercise capacity and lipofuscin)and longevity of C.elegans were observed.The expression of autophagy related marker genes(bec-1 and lgg-1)was detected by quantitative real time-polymerase chain reaction(qRT-PCR).Results Compared with the control group,1-NP could significantly reduce the lifespan of C.elegans,and 104 ng/L group had the most significant effect,with a reduction rate of 28.2%.1-NP(10^(4) ng/L)decreased the body length of C.elegans(P<0.05)and the expression levels of key autophagy genes(bec-1 and lgg-1))(P<0.05),increased lipofuscin accumulation in C.elegans(P<0.05).Conclusion1-NP can shorten the lifespan of C.elegans by decreasing the autophagy level in C.elegans.

关 键 词:1-硝基芘 秀丽隐杆线虫 衰老 自噬 脂褐素 

分 类 号:R114[医药卫生—卫生毒理学]

 

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